生长因子诱导的人及二甲基肼诱导的大鼠结直肠肿瘤细胞系的增殖反应。

Growth factor-induced proliferative responses of human and DMH-induced rat colorectal tumour cell lines.

作者信息

Maley M A, Agrez M V, House A K

出版信息

Aust J Exp Biol Med Sci. 1986 Oct;64 ( Pt 5):445-51. doi: 10.1038/icb.1986.47.

DOI:10.1038/icb.1986.47

Abstract

The proliferative response of three human and three 1,2-dimethylhydrazine (DMH)-induced rat colon tumour cell lines to mouse epidermal growth factor (mEGF) and human urogastrone was examined in two in vitro assays (3H-thymidine uptake in liquid microculture and clonogenicity in semi-solid medium). In the 3H-thymidine uptake assay, one of three rat and one of three human colon tumour cell lines, i.e., r237 and LIM1215, were stimulated by mEGF. In the clonogenic assay, mEGF stimulated the one clonogenic rat tumour cell line r113, which did not respond in the 3H-thymidine uptake assay, and the human tumour cell line LIM1215. Urogastrone stimulated LIM1215 in both assay systems. The significance of these in vitro patterns of response to growth stimulatory factors is discussed.

摘要

在两种体外试验（液体微量培养中的³H-胸腺嘧啶核苷摄取和半固体培养基中的克隆形成能力）中，检测了三种人类结肠肿瘤细胞系和三种1,2-二甲基肼（DMH）诱导的大鼠结肠肿瘤细胞系对小鼠表皮生长因子（mEGF）和人尿抑胃素的增殖反应。在³H-胸腺嘧啶核苷摄取试验中，三种大鼠结肠肿瘤细胞系中的一种以及三种人类结肠肿瘤细胞系中的一种，即r237和LIM1215，受到mEGF的刺激。在克隆形成试验中，mEGF刺激了在³H-胸腺嘧啶核苷摄取试验中无反应的一种具有克隆形成能力的大鼠肿瘤细胞系r113以及人类肿瘤细胞系LIM1215。在两种试验系统中，尿抑胃素均刺激了LIM1215。讨论了这些体外对生长刺激因子的反应模式的意义。