Braun M, Heinen E, Cormann N, Kinet-Denoël C, Simar L J
Cell Immunol. 1987 Jun;107(1):99-106. doi: 10.1016/0008-8749(87)90269-3.
Follicular dendritic cells (FDC) are located only inside lymph follicles and are characterized mainly by their capacity to retain high amounts of immune complexes by their Fc or C3b receptors. In this work, we examine the influence of immunoglobulin isotypes and the subset of lymphoid cells (B or T) upon the transfer of immune complexes from lymphocytes to FDC. FDC isolated from mice lymph nodes by enzymatic digestion are able to fix, through Fc receptors, gold-labeled immune complexes presented by lymphoid cells. As demonstrated by electron microscopy, this transfer requires the establishment of close contacts between both cell types. Using different cell selection techniques we show that B lymphoid cells take up immune complexes more efficiently than do T lymphoid cells and transfer a larger number of them to FDC. This transfer mechanism is dependent on the immunoglobulin isotype: immune complexes constituted of IgG2a, IgG2b, and IgG1 isotypes are better transferred to FDC than those constituted of IgG3 and IgM.
滤泡树突状细胞（FDC）仅位于淋巴滤泡内，其主要特征是能够通过Fc或C3b受体保留大量免疫复合物。在本研究中，我们检测了免疫球蛋白同种型以及淋巴细胞亚群（B细胞或T细胞）对免疫复合物从淋巴细胞转移至FDC的影响。通过酶消化从小鼠淋巴结分离出的FDC能够通过Fc受体固定淋巴细胞呈递的金标记免疫复合物。如电子显微镜所示，这种转移需要两种细胞类型之间建立紧密接触。使用不同的细胞分选技术，我们发现B淋巴细胞比T淋巴细胞更有效地摄取免疫复合物，并将更多的免疫复合物转移至FDC。这种转移机制取决于免疫球蛋白同种型：由IgG2a、IgG2b和IgG1同种型构成的免疫复合物比由IgG3和IgM构成的免疫复合物更易转移至FDC。
