Abrahamson M, Grubb A, Olafsson I, Lundwall A
FEBS Lett. 1987 Jun 1;216(2):229-33. doi: 10.1016/0014-5793(87)80695-6.
Recombinant cystatin C producing clones were isolated from a human placenta lambda gt11 cDNA library. The cDNA insert of one of the clones, containing 777 base pairs, encodes the complete mature cystatin C (120 amino acids) and a hydrophobic leader sequence of 26 amino acids, indicating an extracellular function of the inhibitor. The deduced protein sequence confirms the protein sequence of cystatin C isolated from human urine, but differs in one position from the sequence of the cystatin C fragment deposited as amyloid in hereditary cerebral hemorrhage with amyloidosis.
从人胎盘λgt11 cDNA文库中分离出产生重组胱抑素C的克隆。其中一个克隆的cDNA插入片段包含777个碱基对，编码完整的成熟胱抑素C（120个氨基酸）和一个26个氨基酸的疏水前导序列，表明该抑制剂具有细胞外功能。推导的蛋白质序列证实了从人尿液中分离出的胱抑素C的蛋白质序列，但在一个位置上与遗传性脑出血伴淀粉样变性中作为淀粉样蛋白沉积的胱抑素C片段的序列不同。
