Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001, Guangdong, PR China; Department of Pharmacy, The First Naval Force Hospital of Southern Theatre Command, Zhanjiang, 524005, Guangdong, PR China.
Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi'an, 710032, Shaanxi, PR China.
J Ethnopharmacol. 2022 Apr 6;287:114937. doi: 10.1016/j.jep.2021.114937. Epub 2021 Dec 24.
Bolbostemma paniculatum (Maxim.) Franquet (BPF), a kind of Chinese medicine, has been traditionally used in treating mastitis, dysentery, phlegm nuclear, and sore swelling poison.
In current study, we tried to investigate the possible anti-colorectal cancer (CRC) effects of BPF.
The effects of BPF extract on human colon cancer cells HCT-116 and SW-620, and a colitis associated colorectal cancer (CACC) mouse model were evaluated using the method of experimental pharmacology combined with network pharmacology.
The ethyl acetate extract 3 (EA3) of BPF showed the most potent growth inhibitory effect in CRC cells. It could inhibit the clone formation, induce the apoptosis and cell cycle arrest in G1 phase as well as suppress the invasion and migration of CRC cells. And EA3 prevented ICR mice against CACC effectively. Both KEGG and GO analysis indicated that EA3 may inhibit CRC through influencing PI3K/Akt pathway. Results of Western blot analysis and ELISA confirmed that the molecules in the pathway were affected by EA3.
These results demonstrate that EA3 from BPF could suppress the development of CRC through inhibiting the activity of PI3K/Akt pathway.
冬凌草（Maxim.）Franquet（BPF）是一种中药，传统上用于治疗乳腺炎、痢疾、痰核和肿痛毒。
在本研究中，我们试图研究 BPF 对结肠癌（CRC）的可能的治疗作用。
采用实验药理学与网络药理学相结合的方法，评价 BPF 提取物对人结肠癌细胞 HCT-116 和 SW-620 以及结肠炎相关结直肠癌（CACC）小鼠模型的影响。
BPF 的乙酸乙酯提取物 3（EA3）对 CRC 细胞表现出最强的生长抑制作用。它可以抑制 CRC 细胞的克隆形成，诱导细胞凋亡和 G1 期细胞周期停滞，并抑制 CRC 细胞的侵袭和迁移。并且 EA3 有效预防 ICR 小鼠发生 CACC。KEGG 和 GO 分析均表明，EA3 可能通过影响 PI3K/Akt 通路抑制 CRC。Western blot 分析和 ELISA 的结果证实了该通路中的分子受 EA3 的影响。
这些结果表明，BPF 的 EA3 可以通过抑制 PI3K/Akt 通路的活性来抑制 CRC 的发展。
