Hanssens L, Verachtert H
J Bacteriol. 1976 Mar;125(3):829-36. doi: 10.1128/jb.125.3.829-836.1976.
Mitochondria of the yeastlike fungus Moniliella tomentosa oxidize reduced nicotinamide adenine dinucleotide, reduced nicotinamide adenine dinucleotide phosphate, succinate, isocitrate, and lactate. These oxidations are completely inhibited by cyanide or antimycin A in mitochondria isolated from cells grown in the standard medium. On the other hand, the oxidation of all substrates, except lactate, is almost completely insensitive to cyanide or antimycin A in mitochondria from cells grown in the presence of ethidium bromide. In this instance, the oxidation is mainly mediated by an alternate oxidase which can be blocked by salicyl hydroxamic acid. The alternate oxidase can be specifically stimulated by adenosine 5'-monophosphate and this provides a new method for the characterization of the alternate oxidase in mitochondria of M. tomentosa.
类酵母真菌棉状丛赤壳菌的线粒体可氧化还原型烟酰胺腺嘌呤二核苷酸、还原型烟酰胺腺嘌呤二核苷酸磷酸、琥珀酸、异柠檬酸和乳酸。在从标准培养基中生长的细胞分离得到的线粒体中,这些氧化反应被氰化物或抗霉素A完全抑制。另一方面,在溴化乙锭存在下生长的细胞的线粒体中,除乳酸外,所有底物的氧化对氰化物或抗霉素A几乎完全不敏感。在这种情况下,氧化主要由交替氧化酶介导,水杨基羟肟酸可阻断该酶。交替氧化酶可被5'-单磷酸腺苷特异性刺激,这为棉状丛赤壳菌线粒体中交替氧化酶的表征提供了一种新方法。