Cárdenas-Laverde Diego, Barbosa-Cornelio Ricardo, Coy-Barrera Ericsson
Bioorganic Chemistry Laboratory, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cajicá 250247, Colombia.
Plants (Basel). 2021 Nov 24;10(12):2563. doi: 10.3390/plants10122563.
Plants produce various compounds as defensive barriers to naturally control fungal diseases. Among them, vascular wilt caused by is one of the most destructive diseases in crops, causing relevant economic losses. The application of synthetic fungicides is the most used management for this disease. However, this kind of method also involves adverse environmental impacts. Therefore, alternative methods are continuously being developed as a strategy to be involved in integrated pest management programs. Thus, as part of our research on antifungals of plant origin, a group of botanical extracts was assessed for the respective inhibitory effect on mycelium and conidia of . Mycelial growth inhibition was measured in 12-well plates containing amended semi-solid medium, whereas conidial susceptibility was determined through microdilution. The identification of the bioactive compounds among test extracts was performed using an indirect approach, consisting of the integration of chemical composition and antifungal activity datasets through single- orthogonal partial least squares (OPLS) regression. Results showed that extract was the most potent mycelial growth inhibitor whereas exhibited the best effect on conidia susceptibility. The active compounds identified through statistical integration and subsequent isolation were piperaduncin C, asebogenin and (-)-methyllinderatin. These findings indicated that the integrative, indirect approach is useful for the identification of bioactive metabolites from botanical extracts to be further used as biological protective agents against this phytopathogen.
植物产生各种化合物作为防御屏障以自然控制真菌病害。其中，由[病原菌名称缺失]引起的维管束枯萎病是农作物中最具破坏性的病害之一，会造成相关经济损失。合成杀菌剂的应用是针对这种病害最常用的防治方法。然而，这种方法也会对环境产生不利影响。因此，作为综合虫害管理计划的一部分，替代方法正在不断被开发。因此，作为我们对植物源抗真菌剂研究的一部分，对一组植物提取物对[病原菌名称缺失]的菌丝体和分生孢子的抑制作用进行了评估。在含有改良半固体培养基的12孔板中测量菌丝体生长抑制，而通过微量稀释法测定分生孢子的敏感性。使用间接方法对测试提取物中的生物活性化合物进行鉴定，该方法包括通过单正交偏最小二乘法（OPLS）回归整合化学成分和抗真菌活性数据集。结果表明，[提取物名称缺失]提取物是最有效的菌丝体生长抑制剂，而[提取物名称缺失]对分生孢子敏感性表现出最佳效果。通过统计整合和后续分离鉴定出的活性化合物为胡椒达因C、卫矛醇和（-）-甲基乌药碱。这些发现表明，这种综合的间接方法对于从植物提取物中鉴定生物活性代谢物很有用，这些代谢物可进一步用作针对这种植物病原体的生物保护剂。
