Bioorganic Chemistry Laboratory, Facultad de Ciencias Básicas y Aplicadas, Universidad Militar Nueva Granada, Cajicá 250247, Colombia.
Molecules. 2022 Apr 29;27(9):2832. doi: 10.3390/molecules27092832.
plants are well-recognized due to their significant alkaloid content, which has made them the subject of several studies. However, the lack of chemical and biological information on the Colombian species remains a fact. Therefore, the alkaloidal fractions from the leaves of L. obtained by conventional solvent and ultrasound-assisted extraction (CSE and UAE, respectively) at different time frames were analyzed. Sparteine () was the main component in all cases; however, its relative abundance showed large variability, ranging from 64.7% to 80.6%. Minor constituents were also affected by the extraction conditions. In general, prolonged times gave a higher proportion of alkaloids under CSE, while only a slight decrease was observed under UAE. Both the method and extraction time appeared to equally affect the ratios of particular alkaloids, leading to variations in their effect on the mycelial growth of . Holistic analysis through multiple-covariate statistical methods as an approach to integrating chemical and bioactivity datasets allowed inferring the compounds most likely responsible for the changes in mycelial growth inhibition. 13α-Hydroxylupanine () might represent a promising compound to be included in further studies against this phytopathogen.
植物因其显著的生物碱含量而广为人知，这使得它们成为了多项研究的对象。然而，哥伦比亚特有物种的化学成分和生物信息仍然匮乏。因此，分别采用常规溶剂提取（CSE）和超声辅助提取（UAE）在不同时间框架下从该植物的叶片中提取的生物碱部分进行了分析。在所有情况下，斯帕丁（sparteine）都是主要成分；然而，其相对丰度存在很大的可变性，范围从 64.7%到 80.6%。次要成分也受到提取条件的影响。一般来说，在 CSE 下，延长时间会导致更高比例的生物碱，而在 UAE 下则观察到轻微下降。提取方法和时间似乎对等影响特定生物碱的比例，导致它们对菌丝生长抑制的影响发生变化。通过多变量统计方法进行整体分析，作为整合化学和生物活性数据集的一种方法，可以推断出最有可能导致菌丝生长抑制变化的化合物。13α-羟基羽扇豆碱（13α-hydroxylupanine）可能是对抗这种植物病原菌的有前途的化合物。
