Logtenberg T, Kroon A, Gmelig-Meyling F H, Ballieux R E
Eur J Immunol. 1987 Jun;17(6):855-9. doi: 10.1002/eji.1830170619.
The purpose of our study was to investigate the (auto)antibody repertoire of human tonsil B cells. To this end, unseparated tonsil B cells and "large" and "small" tonsil B cells, obtained after fractionation on a discontinuous Percoll gradient, were fused with the (human X mouse) heterohybridoma K6H6/B5. The IgM antibodies secreted by individual clones were analyzed in spot-enzyme-linked immunosorbent assay for reactivity against a panel of 5 autoantigens and 1 xenoantigen. The results show that both "mono-" and "multispecific" (auto)antibody-secreting clones can be obtained by somatic hybridization of unseparated, "small" and "large" tonsil B cells. The patterns of IgM reactivities in these three collections of hybridomas did not differ significantly. Inhibition experiments revealed that "multispecific" antibodies can bind with high affinity to more than one (auto)antigen. The results of these experiments are discussed in relation to the state of activation of tonsil B lymphocytes.
我们研究的目的是调查人扁桃体B细胞的（自身）抗体库。为此，将未分离的扁桃体B细胞以及在不连续Percoll梯度上分级分离后获得的“大”、“小”扁桃体B细胞与（人×小鼠）异源杂交瘤K6H6/B5进行融合。通过斑点酶联免疫吸附测定法分析各个克隆分泌的IgM抗体与一组5种自身抗原和1种异种抗原的反应性。结果表明，通过未分离的、“小”和“大”扁桃体B细胞的体细胞杂交均可获得分泌“单特异性”和“多特异性”（自身）抗体的克隆。这三组杂交瘤中IgM反应模式没有显著差异。抑制实验表明，“多特异性”抗体可与不止一种（自身）抗原高亲和力结合。结合扁桃体B淋巴细胞的激活状态对这些实验结果进行了讨论。
