Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Thailand.
Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai, Thailand.
Pharm Biol. 2022 Dec;60(1):96-107. doi: 10.1080/13880209.2021.2018470.
Many natural extracts have been shown to minimize the toxicity of doxorubicin (Dox). Low piperine L. (Piperaceae) extract (PFPE) is a natural extract containing many types of antioxidants that may reduce Dox toxicities.
To evaluate the effect of PFPE in attenuating the side effects of Dox.
Tumour-bearing Sprague Dawley rats were divided into five groups including normal, vehicle, 100 mg/kg BW of PFPE plus 2 mg/kg BW of Dox (P100 + Dox), 100 mg/kg BW of PFPE plus 2 mg/kg BW of Dox (P200 + Dox) and Dox. Rats were treated with Dox and/or PFPE three times/week for 4 weeks. Tumour burden, blood parameters, weight of internal organs and immunological data were investigated.
The addition of 200 mg/kg PFPE significantly restored the levels of AST from 174.60 ± 45.67 U/L in the Dox group near to normal levels at 109.80 ± 4.99 U/L. The combination of PFPE and Dox also decreased the levels of CXCL7, TIMP-1, sICAM-1 and l-selectin about 1.4-1.6-fold compared to Dox group. Feeding rats with 200 mg/kg BW of PFPE combination with Dox slightly increased Th1 from 161.67 ± 14.28 cells in Dox group to 200.75 ± 5.8 cells meanwhile suppressed Treg from 3088 ± 78 cells in Dox to 2561 ± 71 cells.
This study showed that PFPE ameliorated Dox toxicity in many aspects indicating the role of antioxidant and other substances in the extract on toxicity attenuation. This suggested the using of PFPE may be valuable for Dox treated patients.
许多天然提取物已被证明可降低多柔比星（阿霉素）的毒性。低胡椒碱 L.（胡椒科）提取物（PFPE）是一种天然提取物，含有许多类型的抗氧化剂，可能减轻阿霉素的毒性。
评估 PFPE 减轻阿霉素副作用的作用。
荷瘤 Sprague Dawley 大鼠分为五组，包括正常组、对照组、100mg/kg BW PFPE 加 2mg/kg BW 阿霉素（P100+Dox）组、100mg/kg BW PFPE 加 2mg/kg BW 阿霉素（P200+Dox）组和阿霉素组。每周三次给大鼠用阿霉素和/或 PFPE 治疗 4 周。研究肿瘤负担、血液参数、内脏器官重量和免疫数据。
添加 200mg/kg PFPE 可使阿霉素组的 AST 水平从 174.60±45.67U/L 恢复到接近正常水平的 109.80±4.99U/L。PFPE 与阿霉素联合使用还可使 CXCL7、TIMP-1、sICAM-1 和 l-选择素的水平降低约 1.4-1.6 倍。与阿霉素组相比，200mg/kg BW PFPE 联合阿霉素喂养大鼠可使 Th1 从阿霉素组的 161.67±14.28 个细胞增加到 200.75±5.8 个细胞，同时将 Treg 从阿霉素组的 3088±78 个细胞减少到 2561±71 个细胞。
本研究表明，PFPE 在多个方面改善了阿霉素的毒性，表明提取物中的抗氧化剂和其他物质在减轻毒性方面的作用。这表明 PFPE 的使用可能对接受阿霉素治疗的患者有价值。
