el Ridi R, Wahby A F, Saad A H, Soliman M A
Immunobiology. 1987 Mar;174(2):177-89. doi: 10.1016/S0171-2985(87)80037-2.
Thymocytes and splenocytes (SC) of adult snakes, Spalerosophis diadema, responded to concanavalin A (Con A) in vitro by strong proliferation during the spring and autumn seasons. Con A-mediated mitogenesis was, however, abrogated in summer and winter. Conditioned medium (CM) collected from snake SC cultures stimulated with Con A in spring or autumn could enhance the Con A summer and winter responses and support the proliferation of splenic lymphoblasts. Gel filtration of native CM on Sephadex G-100 revealed the presence of two biologically active peaks of molecular weight 39-42 and 15 KD. However, only one peak of activity corresponding to molecular weight (m.w.) of 14-15 KD was observed when CM was subjected to analysis by sodium dodecyl sulphate gel electrophoresis (SDS-PAGE). The active molecular forms exhibited isoelectric points of 5.5-5.8 and 6.4-6.6. The findings suggest that Con A activation of snake lymphocytes in optimal seasonal conditions is associated with the secretion of a lymphokine analogous to the interleukin 2 (IL 2) of endothermic vertebrates.
成年蛇类斯氏沙蟒（Spalerosophis diadema）的胸腺细胞和脾细胞在春秋季节体外对刀豆球蛋白A（Con A）有强烈增殖反应。然而，在夏季和冬季，Con A介导的有丝分裂被抑制。从春秋季用Con A刺激的蛇脾细胞培养物中收集的条件培养基（CM）可增强夏季和冬季对Con A的反应，并支持脾淋巴母细胞的增殖。用葡聚糖G-100对天然CM进行凝胶过滤显示存在分子量为39 - 42和15 KD的两个生物活性峰。然而，当CM通过十二烷基硫酸钠凝胶电泳（SDS-PAGE）进行分析时，仅观察到一个对应分子量（m.w.）为14 - 15 KD的活性峰。活性分子形式的等电点为5.5 - 5.8和6.4 - 6.6。这些发现表明，在最佳季节条件下Con A对蛇淋巴细胞的激活与一种类似于恒温脊椎动物白细胞介素2（IL 2）的淋巴因子的分泌有关。
