Department of Biological Sciences, Munster Technological University, Rossa Ave, Bishopstown, Cork, Ireland.
APC Microbiome Ireland, University College Cork, College Road, Cork, Ireland.
Pathog Dis. 2022 Jan 7;79(9). doi: 10.1093/femspd/ftab055.
Campylobacter fetus can cause intestinal and systemic disease in humans and are well-established veterinary and economic pathogens. We report the complete genomic sequences of two C. fetus subsp. fetus (Cff) isolates recovered in 2017 (CITCf01) and 2018 (CITCf02) from a case of recurrent prosthetic valve endocarditis. Both were capable of growth aerobically. Their genomes were found to be highly conserved and syntenic with 99.97% average nucleotide identity (ANI) while differences in their respective sap loci defined the temporal separation of their genomes. Based on core genome phylogeny and ANI of 83 Cff genomes belonging to the previously described human-associated Cff lineage, CITCf01 and CITCf02 grouped in a clade of 11 sequence type (ST)3 Cff (including the Cff type strain NCTC 10842T). CITCf01 and CITCf02 were marked for their lack of unique genomic features when compared to isolates within the subspecies and the type strain in particular. We identified point mutations in oxidative stress response genes, among others, that may contribute to aerobiosis. We report a case of Cff causing relapsed prosthetic valve endocarditis and we highlight the sap island as a polymorphic site within the genetically stable ST3 lineage, central to pathogenicity.
胎儿弯曲菌可引起人类的肠道和全身疾病，是一种公认的兽医和经济病原体。我们报告了两株胎儿弯曲菌亚种胎儿（Cff）分离株的完整基因组序列，它们于 2017 年（CITCf01）和 2018 年（CITCf02）从一例复发性人工瓣膜心内膜炎病例中分离得到。这两株菌都能够在有氧条件下生长。它们的基因组高度保守，与 99.97%的平均核苷酸同一性（ANI）一致，而它们各自sap 基因座的差异定义了它们基因组的时间分离。基于核心基因组系统发育和先前描述的与人类相关的 Cff 谱系中 83 个 Cff 基因组的 ANI，CITCf01 和 CITCf02 分为 11 个序列型（ST）3 Cff （包括 Cff 模式株 NCTC 10842T）的一个分支。与亚种内的分离株和特别是模式株相比，CITCf01 和 CITCf02 缺乏独特的基因组特征。我们在氧化应激反应基因等基因中发现了点突变，这些突变可能有助于需氧生长。我们报告了一例 Cff 引起复发性人工瓣膜心内膜炎的病例，并强调了 sap 岛作为遗传稳定的 ST3 谱系中的多态性位点，是致病性的关键。
