Falanga V, Tiegs S L, Alstadt S P, Roberts A B, Sporn M B
J Invest Dermatol. 1987 Jul;89(1):100-4. doi: 10.1111/1523-1747.ep12580445.
Transforming growth factor-beta (TGF-beta) has been found in all cells examined thus far, and has been shown to play an important role in inflammation and connective tissue formation. We now report that TGF-beta, alone or in combination with epidermal growth factor (EGF), led to a preferential increase in glycosaminoglycan synthesis by cultures of dermal fibroblasts from patients with progressive systemic sclerosis (PSS) when compared with normal fibroblasts (p less than 0.001). Transforming growth factor-beta increased collagen synthesis to the same extent in both PSS and normal fibroblasts, whereas EGF had no stimulatory activity on collagen synthesis. The addition of EGF to cultures incubated with TGF-beta led to a decrease in collagen synthesis compared with the effect seen with TGF-beta alone (p less than 0.02). These studies suggest that TGF-beta may play an important role in the accumulation of connective tissue seen in PSS and that the combined action of multiple growth factors may modulate the synthetic activity of human dermal fibroblasts.
迄今为止，在所有已检测的细胞中均发现了转化生长因子-β（TGF-β），并且已证明其在炎症和结缔组织形成中发挥重要作用。我们现在报告，与正常成纤维细胞相比，单独使用TGF-β或与表皮生长因子（EGF）联合使用时，进行性系统性硬化症（PSS）患者的真皮成纤维细胞培养物中糖胺聚糖合成会优先增加（p<0.001）。TGF-β在PSS成纤维细胞和正常成纤维细胞中均使胶原蛋白合成增加到相同程度，而EGF对胶原蛋白合成没有刺激活性。与单独使用TGF-β的效果相比，在与TGF-β一起培养的培养物中添加EGF会导致胶原蛋白合成减少（p<0.02）。这些研究表明，TGF-β可能在PSS中所见的结缔组织积累中起重要作用，并且多种生长因子的联合作用可能调节人真皮成纤维细胞的合成活性。
