State Institution «National Research Center for Radiation Medicine of the National Academy of MedicalSciences of Ukraine», 53 Yuriia Illienka Str., Kyiv, 04050, Ukraine.
Probl Radiac Med Radiobiol. 2021 Dec;26:297-308. doi: 10.33145/2304-8336-2021-26-297-308.
to establish the relationship between quantitative and qualitative parameters of peripheral blood cells(lymphocytes, neutrophilic granulocytes, monocytes, platelets) depending on the type of somatic diseases andannual internal radiation doses from 137Cs in children - residents of radiologically contaminated territories in thelate period after the Chornobyl Nuclear Power Plant (ChNPP) accident.
There were 175 children included in the study comprising residents of radiologically con-taminated territories (n = 79) aged from 4 to 18 years. Annual internal radiation doses in children from 137Cs rangedfrom 0.004 to 0.067 mSv. Certain blood parameters were assessed in a comparative mode in children having got theradiation doses up to 0.01 mSv and higher. The comparison group (n = 96) included children living in settlementsnot attributed to the radiologically contaminated ones. Incidence and type of somatic diseases and its impact onquantitative and qualitative changes in blood parameters (i.e. lymphocyte, neutrophilic granulocyte, monocyte, andplatelet count) were studied. The cell size, state of nucleus, membranes and cytoplasm, signs of proliferative anddegenerative processes were taken into account.
Incidence and type of somatic diseases in children did not depend on the annual internal radiation dose.Number of cases of monocytosis was significantly higher among the children exposed to ionizing radiation than inthe comparison group (16.6 % vs. 7.3 %). There were, however, no correlation between these changes and radiationdoses. Number of activated blood monocytes with cytoplasmic basophilia and residues of nucleoli in nuclei washigher in individuals with internal radiation doses > 0.01 mSv. A direct correlation between the qualitative param-eters of monocytes and internal radiation doses was established (rs = 0.60; р < 0.001), as well as a direct correlationof different strength between qualitative parameters of blood cells, indicating their unidirectional pattern depend-ing on the somatic morbid conditions. Regardless of annual internal radiation dose, there was an increase in thenumber of degenerative and aberrant cells vs. the comparison group (р < 0.05), which could be due to the role ofnon-radiation factors.
Results of the assessment of quantitative and qualitative parameters of peripheral blood cells reflect-ed the state of morbid conditions in children and are of a diagnostic value. The identified dose-dependent changesin monocyte lineage of hematopoiesis may be the markers of impact of long-term radionuclide incorporation withfood in children living in environmentally unfavorable conditions after the ChNPP accident.
确定儿童外周血细胞（淋巴细胞、中性粒细胞、单核细胞、血小板）的定量和定性参数与躯体疾病类型以及 137Cs 的年体内辐射剂量之间的关系，这些儿童是切尔诺贝利核电站事故后期放射性污染地区的居民。
本研究纳入了 175 名儿童，其中包括 79 名年龄在 4 至 18 岁的放射性污染地区居民。儿童体内 137Cs 的年辐射剂量范围为 0.004 至 0.067mSv。在比较模式下评估了接受辐射剂量低于 0.01mSv 和更高剂量的儿童的某些血液参数。对照组（n=96）包括居住在非放射性污染地区的儿童。研究了躯体疾病的发病率和类型及其对血液参数（即淋巴细胞、中性粒细胞、单核细胞和血小板计数）的定量和定性变化的影响。考虑了细胞大小、核状态、膜和细胞质、增殖和退行性过程的迹象。
儿童躯体疾病的发病率和类型与年体内辐射剂量无关。暴露于电离辐射的儿童单核细胞增多症的病例数明显高于对照组（16.6%比 7.3%）。然而，这些变化与辐射剂量之间没有相关性。体内辐射剂量>0.01mSv 的个体中，具有细胞质嗜碱性和核仁残留物的活化血单核细胞数量较高。单核细胞的定性参数与体内辐射剂量之间存在直接相关性（rs=0.60；p<0.001），以及血液细胞不同强度的定性参数之间存在直接相关性，表明它们存在单向模式，取决于躯体病态条件。无论体内年辐射剂量如何，与对照组相比，退化和异常细胞的数量都会增加（p<0.05），这可能是由于非辐射因素的作用。
外周血细胞的定量和定性参数评估结果反映了儿童病态状况，并具有诊断价值。造血单核细胞系中发现的剂量依赖性变化可能是切尔诺贝利核电站事故后生活在环境不利条件下的儿童长期放射性核素摄入影响的标志物。
