瑞香素通过调控 NF-κB 信号通路抑制 PDGF-BB 诱导的气道平滑肌细胞增殖和迁移。
Scopoletin inhibits PDGF-BB-induced proliferation and migration of airway smooth muscle cells by regulating NF-κB signaling pathway.
机构信息
Department of Pediatrics, Chengdu Wenjiang Maternal and Child Health Hospital, Chengdu, Sichuan Province, China.
Department of Pediatrics, School of Medical and Life Sciences, Reproductive & Women-Children Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China;
出版信息
Allergol Immunopathol (Madr). 2022 Jan 1;50(1):92-98. doi: 10.15586/aei.v50i1.517. eCollection 2022.
BACKGROUND
Asthma is a common chronic inflammatory disease of the airway, and airway remodeling and the proliferation mechanism of airway smooth muscle cells (ASMCs) is of great significance to combat this disease.
OBJECTIVE
To assess possible effects of scopoletin on asthma and the potential signaling pathway.
MATERIALS AND METHODS
ASMCs were treated PDGF-BB and scopoletin and subjected to cell viability detection by CCK-8 assay. Cell migration of ASMCs was determined by a wound closure assay and transwell assay. The protein level of MMP2, MMP9, calponin and α-SMA were measured using western blot. The levels of NF-κB signaling pathway were detected by Western blotting.
RESULTS
Scopoletin inhibited proliferation of PDGF-BB - induced ASMCs. Also it suppressed the migration and invasion of PDGF-BB - induced ASMCs. We further showed that Scopoletin regulated phenotypic transition of ASMCs. Mechanically, Scopoletin inhibited proliferation and invasion of ASMCs by regulating NF-κB signaling pathway.
CONCLUSIONS
We therefore thought Scopoletin could serve as a promising drug for the treatment of asthma.
背景
哮喘是一种常见的气道慢性炎症性疾病，气道重塑和气道平滑肌细胞（ASMCs）的增殖机制对治疗这种疾病具有重要意义。
目的
评估山麦冬苷对哮喘的可能作用及其潜在的信号通路。
材料和方法
用 PDGF-BB 和山麦冬苷处理 ASMCs，并用 CCK-8 法检测细胞活力。通过划痕闭合测定法和 Transwell 测定法测定 ASMCs 的迁移。用 Western blot 测定 MMP2、MMP9、钙调蛋白和 α-SMA 的蛋白水平。通过 Western blot 检测 NF-κB 信号通路的水平。
结果
山麦冬苷抑制 PDGF-BB 诱导的 ASMCs 增殖。它还抑制 PDGF-BB 诱导的 ASMCs 的迁移和侵袭。我们进一步表明，山麦冬苷调节 ASMCs 的表型转化。在机制上，山麦冬苷通过调节 NF-κB 信号通路来抑制 ASMCs 的增殖和侵袭。
结论
因此，我们认为山麦冬苷可以作为一种有前途的治疗哮喘的药物。
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