Institute of TCM and Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan, P.R. China.
MOE Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University, Wuhan, P. R. China.
Nat Prod Res. 2022 Sep;36(17):4371-4378. doi: 10.1080/14786419.2021.1993216. Epub 2021 Dec 29.
Four flavonoid glycosides containing coumaroyl or feruloyl groups were isolated from the male flowers of L., and compounds and were identified as novel compounds. The inhibitory activities against α-glucosidase were investigated by docking studies, assays and kinetic studies. The docking results showed that all compounds mainly formed hydrogen-bond and π-π-stacking interactions with α-glucosidase. Compound had the lowest binding energy and maximum number of hydrogen bonds. Subsequently, the assays showed that compound exhibited the strongest inhibitory potency. Finally, the kinetic studies indicated the inhibitory mode of compounds - against α-glucosidase were mixed types of competitive and non-competitive. Together, these findings suggested that the isolated flavonoid glycosides in this study, especially compound , have potential as α-glucosidase inhibitors.
从 的雄花中分离得到了 4 个含有香豆酰基或阿魏酰基的黄酮糖苷，其中化合物 和 被鉴定为新化合物。通过对接研究、 试验和动力学研究考察了它们对 α-葡萄糖苷酶的抑制活性。对接结果表明，所有化合物主要与 α-葡萄糖苷酶形成氢键和 π-π 堆积相互作用。化合物 具有最低的结合能和最多的氢键。随后， 试验表明化合物 表现出最强的抑制活性。最后，动力学研究表明化合物 - 对 α-葡萄糖苷酶的抑制模式为混合型的竞争和非竞争。综上所述，本研究中分离得到的黄酮糖苷，尤其是化合物 ，可能具有作为 α-葡萄糖苷酶抑制剂的潜力。
