Li Xinghao, Song Nan, Zhang Heng
College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, China.
PeerJ. 2021 Dec 9;9:e12169. doi: 10.7717/peerj.12169. eCollection 2021.
The Coccinellidae are one of the most familiar beetle families, the ladybirds. Despite the great ecological and economic significance, the phylogenetic relationships of Coccinellidae remain poorly understood. One of the reasons is that the sequenced mitogenomes available for this family are very limited. We sequenced complete or nearly complete mitogenomes from seven species of the tribe Coccinellini with next-generation sequencing. All species have the same gene content and gene order as the putatively ancestral insect mitogenome. A large intergenic spacer region (> 890 bp) was found located between and . The potential for using secondary structures of the large and small ribosomal subunits for phylogenetic reconstruction was predicted. The phylogenetic relationships were explored through comparative analyses across more than 30 coccinellid species. We performed phylogenetic analyses with both concatenation methods (Maximum Likelihood and Bayesian Inference) and multispecies coalescent method (ASTRAL). Phylogenetic results strongly supported the monophyly of Coccinellidae. Within Coccinellidae, the Epilachnini and the Coccinellini including Halyziini were monophyletic, while the Scymnini and Coccidulini were non-monophyletic.
瓢虫科是最为人熟知的甲虫科之一，即瓢虫。尽管具有重大的生态和经济意义，但瓢虫科的系统发育关系仍知之甚少。原因之一是该科可用的已测序线粒体基因组非常有限。我们利用下一代测序技术对瓢虫族7个物种的完整或近乎完整的线粒体基因组进行了测序。所有物种的基因含量和基因顺序与假定的祖先昆虫线粒体基因组相同。在[具体基因1]和[具体基因2]之间发现了一个大的基因间隔区（> 890 bp）。预测了利用大、小核糖体亚基二级结构进行系统发育重建的潜力。通过对30多种瓢虫科物种的比较分析探索了系统发育关系。我们使用串联方法（最大似然法和贝叶斯推断）和多物种溯祖法（ASTRAL）进行了系统发育分析。系统发育结果有力地支持了瓢虫科的单系性。在瓢虫科内，食植瓢虫亚科以及包括隐胫瓢虫亚科在内的瓢虫亚科是单系的，而小毛瓢虫亚科和红瓢虫亚科则不是单系的。
