Takahashi W Y, Galvão C W, Urrea-Valencia S, Gonçalves D R P, Hyeda D, Caires E F, Etto R M
Microbial Molecular Biology Laboratory, State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil.
Soil Fertility Laboratory, State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil.
Lett Appl Microbiol. 2022 Apr;74(4):604-612. doi: 10.1111/lam.13645. Epub 2022 Jan 17.
The use of Azospirillum brasilense as a crop inoculant has increased in recent years. Thus, the compatibility of the inoculation technology with seed treatments using pesticides needs to be evaluated. In this study, we evaluated the effect of an insecticide and fungicide formulation on A. brasilense strain FP2 population by culturing and culture-independent approaches. In addition, we evaluated the impact of these pesticides on the ability of A. brasilense to promote plant growth by monitoring biometric traits (root and shoot dry mass and length) of wheat grown in Greenhouse conditions. Seed pesticide dressings, mainly fungicide, led to a significant mortality of A. brasilense over time. The ability of A. brasilense to promote wheat growth also decreased due to pesticide treatments combined with sowing delay. Considering that pesticides confer fitness advantages to the wheat in field condition, our results suggest that sowing within the first 4 h after inoculation maintain the beneficial effects of A. brasilense on wheat growth promotion. Furthermore, we conclude that inoculation and treatment of seeds with pesticides may be compatible techniques when carried out immediately before sowing.
近年来，巴西固氮螺菌作为一种作物接种剂的使用有所增加。因此，需要评估接种技术与使用农药进行种子处理的兼容性。在本研究中，我们通过培养和非培养方法评估了一种杀虫剂和杀菌剂配方对巴西固氮螺菌菌株FP2种群的影响。此外，我们通过监测温室条件下种植的小麦的生物特征（根和茎的干质量和长度），评估了这些农药对巴西固氮螺菌促进植物生长能力的影响。种子农药处理，主要是杀菌剂，随着时间的推移导致巴西固氮螺菌显著死亡。由于农药处理和播种延迟，巴西固氮螺菌促进小麦生长的能力也有所下降。考虑到农药在田间条件下赋予小麦适应性优势，我们的结果表明，接种后4小时内播种可保持巴西固氮螺菌对小麦生长促进的有益效果。此外，我们得出结论，在播种前立即对接种种子进行农药处理可能是兼容的技术。
