R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
Exp Oncol. 2021 Dec;43(4):312-316. doi: 10.32471/exp-oncology.2312-8852.vol-43-no-4.17010.
SLAMF1/CD150 receptor is aberrantly expressed in malignant hematopoietic cells compared to ubiquitous expression in their normal analogues. However, the data about CD150 expression and function outside the hematopoietic system are limited. The aim of this pilot study was to examine the profile of mRNA expression of CD150 isoforms and the protein topology in highly and low malignant breast (BC) and prostate cancer (PC) cell lines.
The study was performed on BC T47D, MDA-MB-231, ВСС/Р and BC/ML cell lines and PC LNCap, Du-145 and PC-3 cell lines. The quantitative polymerase chain reaction was applied for study of CD150 isoforms mRNA expression and flow cytometry was used for determination of protein localization.
Analyzed BC cell lines did not express CD150 on the cell surface membrane (csCD150), but more than 45% of cells were positive for CD150 cytoplasmic reaction (cyCD150). The cyCD150 expression level in T47D cells of luminal BC subtype was higher than in basal BC cell lines MDA-MB-231, ВСС/Р and BC/ML. The PC cell lines expressed CD150 both on the cell surface and in cytoplasm. The highest number of csCD150 and cyCD150 cells was revealed in less aggressive androgen responsive, non-metastatic LNCap cell line. All studied BC and PC cell lines expressed mRNA of canonical transmembrane mCD150 and novel nCD150 isoforms but with different pattern of prevalence. Soluble CD150 isoform was revealed at the low level only in BCC/P BC cell line and LNCap, PC-3 PC cell lines.
We have shown that BC and PC cell lines differentially expressed multifunctional receptor CD150 at the mRNA and protein levels that may indicate its association with the degree of their malignancy.
与普遍存在于正常类似物中的表达相比，SLAMF1/CD150 受体在恶性造血细胞中异常表达。然而，关于造血系统外 CD150 表达和功能的数据有限。本初步研究的目的是检查高恶性和低恶性乳腺癌 (BC) 和前列腺癌 (PC) 细胞系中 CD150 同工型的 mRNA 表达谱和蛋白质拓扑结构。
该研究在 BC T47D、MDA-MB-231、ВСС/Р 和 BC/ML 细胞系和 PC LNCap、Du-145 和 PC-3 细胞系上进行。定量聚合酶链反应用于研究 CD150 同工型 mRNA 表达，流式细胞术用于测定蛋白质定位。
分析的 BC 细胞系在细胞膜表面 (csCD150) 上不表达 CD150，但超过 45%的细胞对 CD150 细胞质反应 (cyCD150) 呈阳性。腔 BC 亚型 T47D 细胞中的 cyCD150 表达水平高于基底 BC 细胞系 MDA-MB-231、ВСС/Р 和 BC/ML。PC 细胞系在细胞膜表面和细胞质中表达 CD150。在侵袭性较低、雄激素反应性、非转移性 LNCap 细胞系中发现了最多的 csCD150 和 cyCD150 细胞。所有研究的 BC 和 PC 细胞系均表达经典跨膜 mCD150 和新型 nCD150 同工型的 mRNA，但表达模式不同。仅在 BCC/P BC 细胞系和 LNCap、PC-3 PC 细胞系中检测到低水平的可溶性 CD150 同工型。
我们已经表明，BC 和 PC 细胞系在 mRNA 和蛋白质水平上以不同的方式表达多功能受体 CD150，这可能表明其与它们的恶性程度有关。
