dsRNAs 靶向褐飞虱：评估对非靶标有益捕食者的风险。
dsRNAs Targeted to the Brown Planthopper : Assessing Risk to a Non-Target, Beneficial Predator, .
机构信息
State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058 Zhejiang Province, China.
Biological Control of Insects Research Laboratory USDA/Agricultural Research Service, 1503 S. Providence Road, Columbia, Missouri 65203, United States.
出版信息
J Agric Food Chem. 2022 Jan 12;70(1):373-380. doi: 10.1021/acs.jafc.1c05487. Epub 2021 Dec 30.
RNA interference (RNAi) technology is becoming a maturing insect management approach. Before commercial-scale application, however, it is necessary to assess risks to non-target organisms (NTOs). Here, we evaluated the influence of RNAi technology, targeted to the brown planthopper (BPH, , Hemiptera: Delphacidae), a serious pest of Asian rice cropping systems, by dsRNA feeding. Three dsRNA fragments, targeting (ds), (ds), and (ds), which were highly lethal to BPH, were selected to evaluate their effects on an important predator of BPH, (Hemiptera: Miridae). It showed that these three dsRNA fragments posed no risks to at worst-case treatments when fed with high concentrations (10×) dsRNAs. These findings not only establish part of a risk assessment protocol for RNAi-based products on NTOs but also contribute to the development and deployment of new technologies for BPH management.
RNA 干扰（RNAi）技术正成为一种成熟的昆虫管理方法。然而，在大规模商业化应用之前，有必要评估其对非靶标生物（NTOs）的风险。在这里，我们通过 dsRNA 喂养评估了针对亚洲水稻种植系统中一种严重害虫褐飞虱（BPH，半翅目：飞虱科）的 RNAi 技术的影响。选择了三个针对 （ds）、 （ds）和 （ds）的 dsRNA 片段，这些片段对 BPH 具有高度致死性，用于评估它们对 BPH 的一种重要捕食者 （半翅目：盲蝽科）的影响。结果表明，在最坏情况下用高浓度（10×）dsRNA 喂养时，这三个 dsRNA 片段对 没有风险。这些发现不仅为基于 RNAi 的产品对 NTOs 的风险评估建立了一部分方案，而且为 BPH 管理新技术的开发和部署做出了贡献。
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