Han Yu, Meng Jiarong, Chen Jie, Cai Wanlun, Wang Yu, Zhao Jing, He Yueping, Feng Yanni, Hua Hongxia
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China.
College of Life Science and Technology, Huazhong Agricultural University, P.R. China.
PLoS One. 2014 Nov 6;9(11):e112315. doi: 10.1371/journal.pone.0112315. eCollection 2014.
T2A-1 is a newly developed transgenic rice that expresses a synthesized cry2Aa gene driven by the maize ubiquitin promoter. T2A-1 exhibits high resistance against lepidopteran pests of rice. The brown planthopper, Nilapavarta lugens (Stål), is a main nontarget sap-sucking insect pest of rice, and Cyrtorhinus lividipennis (Reuter) is the major predator of the eggs and young nymphs of planthoppers. As C. lividipennis may expose to the Cry2Aa protein via N. lugens, it is therefore essential to assess the potential effects of transgenic cry2Aa rice on this predator. In the present study, three experiments were conducted to evaluate the ecological risk of transgenic cry2Aa rice to C. lividipennis: (1) a direct feeding experiment in which C. lividipennis was fed an artificial diet containing Cry2Aa at the dose of 10-time higher than that it may encounter in the realistic field condition; (2) a tritrophic experiment in which the Cry2Aa protein was delivered to C. lividipennis indirectly through prey eggs or nymphs; (3) a realistic field experiment in which the population dynamics of C. lividipennis were investigated using vacuum-suction. Both direct exposure to elevated doses of the Cry2Aa protein and prey-mediated exposure to realistic doses of the protein did not result in significant detrimental effects on the development, survival, female ratio and body weight of C. lividipennis. No significant differences in population density and population dynamics were observed between C. lividipennis in transgenic cry2Aa and nontransgenic rice fields. It may be concluded that transgenic cry2Aa rice had no detrimental effects on C. lividipennis. This study represents the first report of an assessment continuum for the effects of transgenic cry2Aa rice on C. lividipennis.
T2A - 1是一种新培育的转基因水稻，它表达由玉米泛素启动子驱动的合成cry2Aa基因。T2A - 1对水稻鳞翅目害虫表现出高抗性。褐飞虱Nilapavarta lugens（Stål）是水稻的一种主要非靶标刺吸式害虫，而黑肩绿盲蝽Cyrtorhinus lividipennis（Reuter）是飞虱卵和低龄若虫的主要捕食者。由于黑肩绿盲蝽可能通过褐飞虱接触到Cry2Aa蛋白，因此评估转基因cry2Aa水稻对这种捕食者的潜在影响至关重要。在本研究中，进行了三项实验来评估转基因cry2Aa水稻对黑肩绿盲蝽的生态风险：（1）直接喂食实验，给黑肩绿盲蝽喂食含Cry2Aa的人工饲料，其剂量比实际田间条件下可能遇到的剂量高10倍；（2）三级营养实验，通过猎物卵或若虫将Cry2Aa蛋白间接传递给黑肩绿盲蝽；（3）实际田间实验，使用真空抽吸法调查黑肩绿盲蝽的种群动态。直接暴露于高剂量的Cry2Aa蛋白以及通过猎物介导暴露于实际剂量的该蛋白，均未对黑肩绿盲蝽的发育、存活、雌虫比例和体重产生显著不利影响。在转基因cry2Aa稻田和非转基因稻田中的黑肩绿盲蝽之间，未观察到种群密度和种群动态的显著差异。可以得出结论，转基因cry2Aa水稻对黑肩绿盲蝽没有不利影响。本研究是关于转基因cry2Aa水稻对黑肩绿盲蝽影响的评估连续体的首次报告。
