通过分批萃取从人乳清中纯化乳铁蛋白。
The purification of lactoferrin from human whey by batch extraction.
作者信息
Foley A A, Bates G W
出版信息
Anal Biochem. 1987 Apr;162(1):296-300. doi: 10.1016/0003-2697(87)90040-6.
The isolation of lactoferrin from human whey has been accomplished using a rapid two-step procedure. The lactoferrin is directly adsorbed to cellulose phosphate by batch extraction and eluted by a stepped salt and pH gradient. The major impurity, a low-molecular-weight fraction, is quickly removed by gel filtration. The recovered lactoferrin has a purity of about 96%. The yield of lactoferrin averaged 80%. This method of lactoferrin purification greatly reduces the labor and time required, and the procedure is easily scaled to any volume of starting material.
已通过快速两步法从人乳清中分离出乳铁蛋白。乳铁蛋白通过分批萃取直接吸附到磷酸纤维素上，然后通过逐步的盐和pH梯度洗脱。主要杂质，一种低分子量组分，通过凝胶过滤快速去除。回收的乳铁蛋白纯度约为96%。乳铁蛋白的平均产量为80%。这种乳铁蛋白纯化方法大大减少了所需的人力和时间，并且该程序易于按任何起始原料体积进行扩大。
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