College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province 450000, PR China; Collaborative Innovation Center for Food Production and Safety of Henan Province, Zhengzhou 450002, Henan Province, PR China.
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan Province 450000, PR China.
Syst Appl Microbiol. 2022 Jan;45(1):126291. doi: 10.1016/j.syapm.2021.126291. Epub 2021 Dec 17.
Faba bean (Vicia faba L.) is a major introduced grain-legume crop cultivated in China. In this study, rhizobia that nodulated faba bean grown in soils from three sites in North China (Hebei Province) were isolated and characterized. Firstly, isolates were categorized into genotypes by ribosomal IGS PCR-RFLP analysis, then representatives of the different IGS genotypes were further identified by phylogenetic analyses of 16S rRNA, housekeeping (atpD, recA) and nodulation (nodC) gene sequences. Rhizobial distribution based on the IGS genotype was related to the different soil physicochemical features by redundancy analysis. IGS typing and phylogenetic analyses of 16S rRNA and concatenated housekeeping gene sequences affiliated the 103 rhizobial strains isolated into four Rhizobium species/genospecies. A total of 69 strains of 3 IGS types were assigned to R. sophorae, 20 isolates of 5 IGS types to R. changzhiense and 9 isolates of 3 IGS types to R. indicum. The representative strain of the five remaining isolates (1 IGS type) was clearly separated from all Rhizobium type strains and was most closely related to defined genospecies according to the recently described R. leguminosarum species complex. Rhizobium sophorae strains (67% of total isolates) were common in all sites and shared an identical nodC sequence typical of faba bean symbionts belonging to symbiovar viciae. In this first study of rhizobia nodulating faba bean in Hebei Province, China, R. sophorae was found to be the dominant symbiont in contrast to other countries.
蚕豆（Vicia faba L.）是中国主要引进的粮食豆类作物之一。本研究从中国华北地区（河北省）的三个地点采集的土壤中分离并鉴定了能与蚕豆共生的根瘤菌。首先，通过核糖体 IGS PCR-RFLP 分析将分离株分为基因型，然后通过 16S rRNA、看家（atpD、recA）和结瘤（nodC）基因序列的系统发育分析，对不同 IGS 基因型的代表进行进一步鉴定。基于 IGS 基因型的根瘤菌分布与不同土壤理化特性通过冗余分析相关。IGS 分型和 16S rRNA 及串联看家基因序列的系统发育分析将 103 株根瘤菌菌株分为 4 个根瘤菌种/种。共 69 株 3 种 IGS 型菌株归为 R. sophorae，20 株 5 种 IGS 型菌株归为 R. changzhiense，9 株 3 种 IGS 型菌株归为 R. indicum。其余 5 个 IGS 型代表菌株（1 种 IGS 型）与所有根瘤菌菌株明显分离，与最近描述的 R. leguminosarum 种复合体中定义的种密切相关。R. sophorae 菌株（占总分离株的 67%）在所有地点都很常见，并且具有与属于 symbiovar viciae 的蚕豆共生体典型的相同 nodC 序列。在中国河北省首次研究能与蚕豆共生的根瘤菌时，发现 R. sophorae 是优势共生体，与其他国家不同。
