Department of Obstetrics and Gynaecology, Clinical Hospital "Sveti Duh", Zagreb, Croatia; Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia.
BetaPlus Centre for Reproductive Medicine, Zagreb, Croatia.
Pregnancy Hypertens. 2022 Mar;27:69-73. doi: 10.1016/j.preghy.2021.12.009. Epub 2021 Dec 24.
The purpose of this study was to determine if elevated concentration of soluble receptor tunica interna endothelial cell kinase-2 (Tie-2) in the amniotic fluid represent a risk factor for the subsequent development of preeclampsia (PE).
Amniotic fluid samples were collected as a part of routine clinical diagnostics from women referred to clinical care due to genetic indications. A total of 12 women with preeclampsia and 26 normotensive pregnant women were included in the study. Mean gestational age at amniocentesis was 17.92 weeks of pregnancy in preeclampsia and 17.88 in control group, respectively. Concentrations of sTie-2 in the amniotic fluid were determined by a standardized enzyme immunoassay.
Median concentration of Tie-2 in the amniotic fluid of PE patients was lower (median 1.109 ng/ml) compared with normotensive pregnant women (median 1.433 ng/Ml) but the difference was not statistically significant (p = 0.2973). Concentration of sTie-2 in the amniotic fluid did not significantly correlate with maternal age, gestational age at amniocentesis or delivery, as well as weight or length at birth. A difference in the gestational age at delivery in PE patients (mean 37.7 weeks) and normotensive pregnant controls (mean 39.8 weeks) was statistically significant (p = 0.0003). Birth weight and length of children delivered by PE women (mean 2863.3 g and 48.3 cm) were significantly lower compared with normal pregnancies (mean 3591.2 g and 51.4 cm, p = 0.0002 and p = 0.006, respectively).
Our results suggest that amniotic fluid concentrations of sTie-2 do not predict development of PE and that further studies on biomarkers as predictors of PE should include other angiogenic biological response modifiers.
本研究旨在确定羊水中可溶性受体内膜内皮细胞激酶-2（Tie-2）浓度升高是否是子痫前期（PE）后续发生的危险因素。
羊水样本作为常规临床诊断的一部分，从因遗传原因就诊的女性中采集。共纳入 12 例子痫前期孕妇和 26 例正常妊娠孕妇。子痫前期组羊膜穿刺术时的平均孕龄为 17.92 周，对照组为 17.88 周。采用标准化酶联免疫吸附法测定羊水 sTie-2 浓度。
PE 患者羊水中 Tie-2 的中位数浓度较低（中位数 1.109ng/ml），与正常妊娠孕妇相比（中位数 1.433ng/ml），但差异无统计学意义（p=0.2973）。羊水 sTie-2 浓度与母亲年龄、羊膜穿刺术时的孕龄或分娩时的体重或身长无显著相关性。PE 患者的分娩孕周（平均 37.7 周）与正常妊娠对照组（平均 39.8 周）有显著差异（p=0.0003）。PE 孕妇所分娩的婴儿体重和身长（均值 2863.3g 和 48.3cm）明显低于正常妊娠（均值 3591.2g 和 51.4cm，p=0.0002 和 p=0.006）。
本研究结果提示，羊水 sTie-2 浓度不能预测 PE 的发生，进一步研究预测 PE 的生物标志物时，应包括其他血管生成生物反应调节剂。
