Department of Microbiology and Food Technology, UTP University of Science and Technology, Bydgoszcz, Poland.
Plant Breeding and Acclimatization Institute - National Research Institute, Bydgoszcz, Poland.
Ann Agric Environ Med. 2021 Dec 29;28(4):595-604. doi: 10.26444/aaem/131799. Epub 2021 Jan 4.
The ability of to create biofilm results in the higher resistance to disinfectants and determines the need to search for effective methods of eradication. The aim of the study was to assess the level of contamination in the environment of a meat processing plant. The sensitivity of tested isolates to various antimicrobials used for disinfection purposes was also estimated.
The samples were taken from raw materials, semi-finished and final products, as well as food contact surfaces inthe production hall and deli meat packaging department. The number of and the effect of eight different biocides on bacteria planktonic forms and biofilm formed on stainless steel and polypropylene was investigated. The effect of blood and albumin on resistance to disinfectants was also analysed.
The prevalence of on food contact surfaces was estimated at 2.93% (10 of 340 swabs taken). The samples of raw and processed products were not contaminated. Various disinfectants reduced the growth of planktonic forms at both tested concentrations 0.5% and 0.1% (irrespective of time exposure). The highest efficacy against biofilm was reported for agents containing hydrogen peroxide. The reduction of bacteria number ranged from 6.93-7.21 log CFU × cm-2, and was dependent on the surface type and time of agent application.
In this study, the effectiveness of various disinfectants against planktonic bacteria and Listeria biofilm was observed. For the majority of disinfectants, the extension of time exposure increased bacteria elimination from the biofilm. The presence of blood resulted in reduction of the antilisterial action of most of the disinfectants applied at low concentrations.
能够形成生物膜，使其对消毒剂具有更高的抵抗力，并决定了需要寻找有效的根除方法。本研究旨在评估肉类加工厂环境中 的污染程度。还评估了测试分离株对用于消毒目的的各种抗菌剂的敏感性。
从原材料、半成品和成品以及生产大厅和熟食包装部门的食品接触面采集样本。调查了 的数量以及八种不同杀生物剂对浮游细菌形式和不锈钢及聚丙烯上形成的生物膜的影响。还分析了血液和白蛋白对消毒剂抗性的影响。
估计食品接触面 的流行率为 2.93%（从 340 个拭子中取 10 个）。未对原料和加工产品的样本进行污染。各种消毒剂在测试浓度 0.5%和 0.1%下均可减少浮游菌的生长（无论暴露时间如何）。含过氧化氢的制剂对 生物膜的效果最高。细菌数量的减少范围为 6.93-7.21 log CFU × cm-2，取决于表面类型和制剂应用时间。
在这项研究中，观察到各种消毒剂对浮游菌和李斯特菌生物膜的有效性。对于大多数消毒剂，延长暴露时间可从生物膜中消除更多的细菌。在低浓度下，血液的存在会降低大多数应用消毒剂的抗李斯特菌作用。
