Faculty of Health Sciences, Pope John Paul II State School of Higher Education in Biała Podlaska, Poland.
Department of Infectious Diseases, Medical University, Lublin, Poland.
Ann Agric Environ Med. 2021 Dec 29;28(4):605-611. doi: 10.26444/aaem/132786. Epub 2021 Feb 11.
The aim of the study was to analyze the concentrations of some serological parameters in patients undergoing antibiotic therapy for LD, and to assess the potential use of IgG anti-C6VlsE in the assessment of treatment effectiveness.
The study involved 21 patients diagnosed with LD. To determine the levels of anti-C6VlsE IgG, metalloproteinases and cytokines, blood was collected three times: before treatment, immediately after treatment, and 3-4 months after treatment.
Immediately after the antibiotic therapy, all the patients in the study group declared improvement in their health. At 3-4 months post-treatment, 50% of patients reported recurrence of the disease symptoms. The anti-C6VlsE IgG concentrations at the analyzed time points exhibited varied dynamics: in 15% of patients, a decrease in the concentration of anti-C6VlsE was observed in the three consecutive serum samples; an increase of anti-C6VlsE IgG was detected in 35% of cases; in 15% of the subjects, the concentration of anti-C6VlsE IgG increased immediately after the antibiotic therapy and declined after 3-4 months, whereas a reverse situation was noted in 10% of the patients.
The presence of high concentrations of anti-C6VlsE IgG is associated with appearance of response to Borreliella spp. infection, which suggests that the parameter can be used in the diagnosis of the active form of borreliosis. However, from a practical point of view, a marker of successful elimination of infection should indicate the effectiveness or failure of antibiotic therapy within a relatively short post-treatment period. The anti-C6VlsE IgG antibodies do not seem to satisfy this provision.
本研究旨在分析接受 LD 抗生素治疗的患者的某些血清学参数浓度，并评估 IgG 抗 C6VlsE 在评估治疗效果中的潜在用途。
本研究纳入了 21 名确诊为 LD 的患者。为了确定 IgG 抗 C6VlsE、金属蛋白酶和细胞因子的水平，在治疗前、治疗后立即和治疗后 3-4 个月采集了三次血液。
在抗生素治疗后，研究组的所有患者均立即宣布病情好转。在治疗后 3-4 个月时，50%的患者报告疾病症状复发。分析时间点的 IgG 抗 C6VlsE 浓度表现出不同的动态：在 15%的患者中，连续三次血清样本中观察到 IgG 抗 C6VlsE 浓度降低；在 35%的病例中检测到 IgG 抗 C6VlsE 的增加；在 15%的患者中，抗生素治疗后 IgG 抗 C6VlsE 浓度立即升高，3-4 个月后下降，而在 10%的患者中则出现相反的情况。
高浓度 IgG 抗 C6VlsE 的存在与对伯氏疏螺旋体感染的反应有关，这表明该参数可用于诊断伯氏疏螺旋体病的活动形式。然而，从实际的角度来看，感染消除的标志物应该在相对较短的治疗后期间内指示抗生素治疗的有效性或失败。IgG 抗 C6VlsE 抗体似乎不符合这一规定。
