杆状病毒多角体蛋白过表达的分子机制。

Molecular mechanism responsible for the hyperexpression of baculovirus polyhedrin.

机构信息

College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Hangzhou 310058, China.

出版信息

Gene. 2022 Mar 10;814:146129. doi: 10.1016/j.gene.2021.146129. Epub 2021 Dec 28.

DOI:10.1016/j.gene.2021.146129

PMID:34971751

Abstract

One of the amazing phenomena in the baculovirus life cycle is the hyperexpression of the very late gene, polyhedrin (polh), causing the production of the occlusion bodies where progeny virions are embedded. However, to date, the molecular mechanism underlying its hyperexpression is not completely elucidated. Considering that, in this review, the mechanism responsible for its hyperexpression from the previous studies up to now was comprehensively summarized from three aspects, namely, the structure characteristics of the polh promoter and transcription regulation, the structure and translation regulation of the polh mRNA, and especially the regulators that influence the expression of polh gene. Moreover, this review will help us obtain a better understanding about the hyperexpression of polh, and also provide guidance for improving the expression efficiency of the foreign proteins by adopting the baculovirus expression vector system.

摘要

杆状病毒生活史中的一个惊人现象是极晚期基因多角体蛋白（polh）的超表达，导致包含子代病毒粒子的包埋体的产生。然而，迄今为止，其超表达的分子机制尚未完全阐明。考虑到这一点，在本综述中，从三个方面综合总结了迄今为止从先前研究中发现的导致其超表达的机制，即 polh 启动子的结构特征和转录调控、polh mRNA 的结构和翻译调控，以及特别影响 polh 基因表达的调控因子。此外，本综述将帮助我们更好地理解 polh 的超表达，并为通过杆状病毒表达载体系统提高外源蛋白的表达效率提供指导。