在中蛋白-基因直系同源物：对祖先共同编码序列的详尽分析，以重新定义之。

Protein-Gene Orthology in : An Exhaustive Analysis to Redefine the Ancestrally Common Coding Sequences.

机构信息

Laboratorio de Ingeniería Genética y Biología Celular y Molecular-Área Virosis de Insectos (LIGBCM-AVI), Instituto de Microbiología Básica y Aplicada, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, Bernal B1876BXD, Buenos Aires, Argentina.

出版信息

Viruses. 2023 Apr 29;15(5):1091. doi: 10.3390/v15051091.

DOI:10.3390/v15051091

PMID:37243176

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10223672/

Abstract

Baculoviruses are entomopathogens that carry large, double-stranded circular DNA genomes and infect insect larvae of Lepidoptera, Hymenoptera and Diptera, with applications in the biological control of agricultural pests, in the production of recombinant proteins and as viral vectors for various purposes in mammals. These viruses have a variable genetic composition that differs between species, with some sequences shared by all known members, and others that are lineage-specific or unique to isolates. Based on the analysis of nearly 300 sequenced genomes, a thorough bioinformatic investigation was conducted on all the baculoviral protein coding sequences, characterizing their orthology and phylogeny. This analysis confirmed the 38 protein coding sequences currently considered as core genes, while also identifying novel coding sequences as candidates to join this set. Accordingly, homology was found among all the major occlusion body proteins, thus proposing that the polyhedrin, granulin and CUN085 genes be considered as the 39th core gene of .

摘要

杆状病毒是一种昆虫病原，携带大型双链环状 DNA 基因组，感染鳞翅目、膜翅目和双翅目的昆虫幼虫，可应用于农业害虫的生物防治、重组蛋白的生产以及作为哺乳动物各种目的的病毒载体。这些病毒的遗传组成具有变异性，在物种之间存在差异，有些序列为所有已知成员共有，而有些则是谱系特异性的或仅限于分离株。基于对近 300 个测序基因组的分析，对所有杆状病毒蛋白编码序列进行了全面的生物信息学研究，对其同源性和系统发育进行了分析。这项分析证实了目前被认为是核心基因的 38 个蛋白编码序列，同时还确定了新的编码序列作为候选基因加入该核心基因集。因此，在所有主要的囊膜蛋白之间发现了同源性，从而提出将多角体蛋白、颗粒体蛋白和 CUN085 基因视为. 的第 39 个核心基因。