Wuxi Hospital of Traditional Chinese Medicine, China; Department of Orthopedics, The Affiliated Wuxi No.2 People's Hospital of Nanjing Medical University, Wuxi, Jiangsu 214000, China.
Center for Circadian Clocks, Soochow University, Suzhou 215123, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Apr;254:109254. doi: 10.1016/j.cbpc.2021.109254. Epub 2021 Dec 29.
In this study, we aimed to assess the developmental toxicity and effects of 5-HMF in zebrafish as a model organism for toxicology studies. To this end, we treated zebrafish embryos with 1-100 μg/ml 5-HMF and observed bone staining, gene expression, and reactive oxygen species levels in order to investigate the toxicological effects of 5-HMF. The results showed that high concentrations of 5-HMF caused increased mortality and deformity rates in zebrafish larvae, inhibited cartilage development, reduced bone mineralization, increased reactive oxygen species levels, and disrupted the expression of genes related to bone development and reactive oxygen species enzyme activity. The antioxidant N-acetyl-l-cysteine partially rescued the toxicological effects caused by the high concentrations of 5-HMF. Overall, these findings showed that high concentrations of 5-HMF induce reactive oxygen species production, leading to developmental toxicity and decreased bone mineralization. Our results provide a reference for understanding the toxic effects of 5-HMF.
在这项研究中，我们旨在评估 5-HMF 在斑马鱼中的发育毒性和作用，斑马鱼作为毒理学研究的模式生物。为此，我们用 1-100μg/ml 的 5-HMF 处理斑马鱼胚胎，观察骨染色、基因表达和活性氧水平，以研究 5-HMF 的毒性作用。结果表明，高浓度的 5-HMF 导致斑马鱼幼虫死亡率和畸形率增加，抑制软骨发育，减少骨矿化，增加活性氧水平，并破坏与骨发育和活性氧酶活性相关的基因表达。抗氧化剂 N-乙酰-l-半胱氨酸部分挽救了高浓度 5-HMF 引起的毒性作用。总的来说，这些发现表明，高浓度的 5-HMF 诱导活性氧的产生，导致发育毒性和骨矿化减少。我们的结果为理解 5-HMF 的毒性作用提供了参考。
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