Chen Peipei, Liu Hao, Xing Zhilin, Wang Yongqiong, Zhang Xiaoping, Zhao Tiantao, Zhang Yunru
School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China.
School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China.
Ecotoxicol Environ Saf. 2022 Jan 15;230:113110. doi: 10.1016/j.ecoenv.2021.113110. Epub 2021 Dec 28.
The cometabolism mechanism of chlorinated hydrocarbon solvents (CHSs) in mixed consortia remains largely unknown. CHS biodegradation characteristics and microbial networks in methanotrophic consortia were studied for the first time. The results showed that all CHSs can efficiently be degraded via cometabolism with a maximum degradation rate of 4.8 mg/(h·g). Chloroalkane and chloroethylene were more easily degraded than chlorobenzenes by methanotrophic consortia, especially nonfully chlorinated aliphatic hydrocarbons, which were converted to Cl with a production rate of 0.29-0.36 mg/(h·g). In addition, the microecological response results indicated that Methylocystaceae (49.0%), Methylomonas (65.3%) and Methylosarcina (41.9%) may be the major functional degraders in methanotrophic consortia. Furthermore, the results of the microbial correlation network suggested that interactive relationships constructed by type I methanotrophs and heterotrophs determined biodegradability. Additionally, PICRUSt analysis showed that CHSs could increase the relative abundance of CHS degradation genes and reduce the relative abundance of methane oxidation genes, which was in good agreement with the experimental results.
混合菌群中氯代烃溶剂（CHSs）的共代谢机制在很大程度上仍然未知。首次研究了甲烷营养菌群中CHS的生物降解特性和微生物网络。结果表明，所有CHSs均可通过共代谢有效降解，最大降解速率为4.8 mg/(h·g)。甲烷营养菌群对氯代烷烃和氯乙烯的降解比对氯苯更容易，尤其是非完全氯化的脂肪烃，其转化为Cl的产率为0.29 - 0.36 mg/(h·g)。此外，微生态响应结果表明，甲基孢囊菌科（49.0%）、甲基单胞菌属（65.3%）和甲基八叠球菌属（41.9%）可能是甲烷营养菌群中的主要功能降解菌。此外，微生物相关网络的结果表明，由I型甲烷营养菌和异养菌构建的相互作用关系决定了生物降解能力。另外，PICRUSt分析表明，CHSs可增加CHS降解基因的相对丰度，降低甲烷氧化基因的相对丰度，这与实验结果高度一致。
