Kuroki M, Tanaka R, Suzuki Y, Moriyama M, Kuwabara Y, Kobayashi S
J Interferon Res. 1987 Jun;7(3):301-11. doi: 10.1089/jir.1987.7.301.
A study was made on the antitumor effect of recombinant murine interferon-beta (rMuIFN-beta) against virus-induced mouse malignant glioma. In in vitro experiments, a dose-dependent and a time-dependent manner were observed for 24 h in the antiproliferative activity of rMuIFN-beta. In in vitro experiments, the growth of subcutaneously transplanted gliomas in mice treated with intraperitoneal administration of rMuIFN-beta was inhibited slightly, whereas, with intratumoral administration, it was inhibited more effectively. In immunological studies, natural killer (NK) cell and cytotoxic T-lymphocyte activities of spleen cells were enhanced with intraperitoneal administration of rMuIFN-beta. The analysis of IFN-induced change in a subpopulation of peripheral blood lymphocytes revealed a decrease of Thy.1,2-positive cells and an increase in the ratio of Lyt.1/Lyt.2-positive cells. But these immunological effects of rMuIFN-beta declined on the 7th day with daily administration. Such hyporeactivity may be noteworthy for the clinical application of IFN.
对重组鼠干扰素-β（rMuIFN-β）抗病毒诱导的小鼠恶性胶质瘤的抗肿瘤作用进行了研究。在体外实验中，观察到rMuIFN-β的抗增殖活性在24小时内呈剂量依赖性和时间依赖性。在体外实验中，腹腔注射rMuIFN-β治疗的小鼠皮下移植胶质瘤的生长略有抑制，而瘤内注射则更有效。在免疫学研究中，腹腔注射rMuIFN-β可增强脾细胞的自然杀伤（NK）细胞和细胞毒性T淋巴细胞活性。对干扰素诱导的外周血淋巴细胞亚群变化的分析显示，Thy.1,2阳性细胞减少，Lyt.1/Lyt.2阳性细胞比例增加。但每日给药时，rMuIFN-β的这些免疫学效应在第7天下降。这种低反应性在干扰素的临床应用中可能值得关注。
