Karasawa M, Yatabe H, Omine M, Maekawa T
J Nutr Sci Vitaminol (Tokyo). 1987 Feb;33(1):21-30. doi: 10.3177/jnsv.33.21.
The growth and DNA synthesis of L1210 mouse leukemia cells were examined under folate- and methionine-deficient conditions. Cell proliferation was dependent on methionine supplementation rather than on folate concentration. The UdR suppression value was abnormally high in the folate-deficient condition. However, it was also high when the methionine was low, despite folate supplementation. In accordance with this, UdR incorporation was significantly improved with various folates by cells grown in low-methionine conditions. Methionine depletion resulted in marked impairment of UdR incorporation regardless of folate concentration. These findings indicate close metabolic interrelations between folate and methionine, which may be relevant to the pathological biochemistry of human megaloblastic anemia.
在叶酸和蛋氨酸缺乏的条件下，对L1210小鼠白血病细胞的生长和DNA合成进行了检测。细胞增殖依赖于蛋氨酸的补充，而非叶酸浓度。在叶酸缺乏的条件下，尿苷抑制值异常高。然而，尽管补充了叶酸，但当蛋氨酸含量低时，该值也很高。据此，在低蛋氨酸条件下生长的细胞，各种叶酸均可显著改善尿苷掺入。无论叶酸浓度如何，蛋氨酸耗竭都会导致尿苷掺入明显受损。这些发现表明叶酸和蛋氨酸之间存在密切的代谢相互关系，这可能与人类巨幼细胞贫血的病理生物化学有关。
