新型猪主动脉补片用于血管重建的细胞相容性分析。
Analysis of the Cytocompatibility of a Novel Porcine Aortic Patch for Vascular Reconstruction.
机构信息
Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany.
BerlinAnalytix GmbH, Berlin, Germany.
出版信息
In Vivo. 2022 Jan-Feb;36(1):63-75. doi: 10.21873/invivo.12677.
BACKGROUND/AIM: Cardiovascular diseases are one of the most common causes of morbidity and mortality in the world. In the case of severe arteriosclerotic damage, surgical treatment is necessary. Although the use of autologous vessels is still considered to be the gold standard, sufficient autologous vessels for transplantation are lacking.
MATERIALS AND METHODS
In the present study, histological examination and in vitro cytotoxicity analysis according to DIN EN ISO 10993-5 were performed on a newly developed porcine vascular graft from a decellularized aorta. A conventional bovine graft was used as control.
RESULTS
The ex vivo-histological analysis revealed the effectiveness of a new purification process on the microstructure and the removal of xenogeneic antigen-bearing structures in the new vessels. Furthermore, cell viability and cytotoxicity assays revealed full cytocompatibility.
CONCLUSION
The novel graft shows no structural damage and gets completely decellularized by the purification process. Superior cytocompatibility, compared with the bovine-derived vascular graft, was demonstrated.
背景/目的：心血管疾病是世界上发病率和死亡率最高的疾病之一。在严重的动脉粥样硬化损伤的情况下，需要进行手术治疗。虽然使用自体血管仍然被认为是金标准，但可用于移植的自体血管却不足。
材料和方法
本研究对一种新开发的去细胞猪主动脉血管移植物进行了组织学检查和根据 DIN EN ISO 10993-5 的体外细胞毒性分析。使用传统的牛血管移植物作为对照。
结果
离体组织学分析显示，新的纯化工艺在微观结构和新血管中异种抗原载体结构的去除方面非常有效。此外，细胞活力和细胞毒性检测显示出完全的细胞相容性。
结论
新型移植物没有结构损伤，并且通过纯化过程完全去细胞化。与牛衍生的血管移植物相比，新型移植物具有更好的细胞相容性。
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