甲状腺手术后大鼠模型气管黏膜内稳态的恢复。
Restoration of Homeostasis in the Tracheal Mucosa After Thyroid Surgery in a Rat Model.
机构信息
Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, College of Medicine, Goyang, Republic of Korea.
Sensory Organ Research Institute, College of Medicine, Dongguk University, Gyengju, Republic of Korea.
出版信息
In Vivo. 2022 Jan-Feb;36(1):161-169. doi: 10.21873/invivo.12687.
BACKGROUND/AIM: This study aimed to investigate the process of homeostatic restoration in the tracheal mucosa (TM) after thyroid surgery.
MATERIALS AND METHODS
Fifty-four rats were divided into normal controls (NC) and three experimental groups: (i) flap elevation (FE), (ii) thyroid exposure (TE), and (iii) thyroid isthmusectomy (TI). Expression of mRNA and proteins of key factors regulating homeostasis were evaluated in the TM obtained 3, 7, and 21 days after thyroid surgery.
RESULTS
Increased mRNA expression of transforming growth factor-β1 (TGF-β1), hypoxia-inducible factor-1α (HIF-1α), and matrix metalloproteinase-9 (MMP-9) were observed 21 days after thyroid surgery in all experimental groups compared to that of NC group.
CONCLUSION
Thyroid surgery leads to an actual increase of TGF-β1, HIF-1α, and MMP-9 expression in the TM. This increased expression of key regulators of homeostatic restoration in the TM lasts for a considerable period of time after surgery, especially if the extent of surgery increased.
背景/目的：本研究旨在探讨甲状腺手术后气管黏膜（TM）的稳态恢复过程。
材料和方法
将 54 只大鼠分为正常对照组（NC）和三个实验组：（i）皮瓣抬高（FE），（ii）甲状腺暴露（TE），和（iii）甲状腺峡部切除术（TI）。在甲状腺手术后 3、7 和 21 天，评估调节稳态的关键因子的 TM 中 mRNA 和蛋白质的表达。
结果
与 NC 组相比，所有实验组在甲状腺手术后 21 天观察到转化生长因子-β1（TGF-β1）、缺氧诱导因子-1α（HIF-1α）和基质金属蛋白酶-9（MMP-9）的 mRNA 表达增加。
结论
甲状腺手术导致 TM 中 TGF-β1、HIF-1α 和 MMP-9 的表达实际增加。TM 中稳态恢复的关键调节因子的这种增加表达在手术后持续相当长的一段时间，尤其是手术范围增加的情况下。
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