Department of Otorhinolaryngology-Head and Neck Surgery, Ilsan Hospital, Dongguk University, Goyang, Republic of Korea
Department of Otorhinolaryngology-Head and Neck Surgery, Ilsan Hospital, Dongguk University, Goyang, Republic of Korea.
In Vivo. 2020 May-Jun;34(3):1133-1140. doi: 10.21873/invivo.11885.
BACKGROUND/AIM: This study aimed to investigate changes in the tracheal mucosa after thyroidectomy, that can be a cause of post-thyroidectomy discomfort.
Forty rats were divided into normal controls and 3 surgical groups: (i) thyroid isthmectomy with cauterization, (ii) isthmectomy by a cold instrument without hemostasis, and (iii) sham (exposure of the trachea and thyroid gland without thyroidectomy by dissection through pretracheal fascia). Animals were euthanized at 1 and 4 weeks. Mucosal edema and glandular hyperplasia were measured. Mucin production and basal cell activities were evaluated by mucin 5AC (MUC5AC) and keratin 5 (KRT5) using immunofluorescence staining.
Larger mucosal areas were observed in all surgical groups at 1 and 4 weeks. More submucosal glandular hyperplasia was noted in the group with isthmectomy without hemostasis. MUC5AC and KRT5 expressions were significantly higher in the surgical groups.
The tracheal mucosa may change after surgery, which could explain postoperative discomfort after thyroidectomy.
背景/目的：本研究旨在探讨甲状腺切除术后气管黏膜的变化，这可能是甲状腺切除术后不适的原因。
将 40 只大鼠分为正常对照组和 3 个手术组：（i）电灼甲状腺峡部切除术，（ii）冷器械甲状腺峡部切除术无止血，和（iii）假手术（通过经气管前筋膜切开暴露气管和甲状腺而不进行甲状腺切除术）。动物在 1 周和 4 周时处死。测量黏膜水肿和腺体增生。通过免疫荧光染色用粘蛋白 5AC（MUC5AC）和角蛋白 5（KRT5）评估粘蛋白产生和基底细胞活性。
在所有手术组中，1 周和 4 周时均观察到更大的黏膜面积。无止血的峡部切除术组中观察到更多的黏膜下腺增生。MUC5AC 和 KRT5 的表达在手术组中明显更高。
气管黏膜在手术后可能会发生变化，这可以解释甲状腺切除术后的术后不适。
