强光短期暴露后家兔眼光毒性的安全性试验。
A Safety Test for Ocular Phototoxicity in the Rabbit After Short-term Exposure to Strong Light.
机构信息
Department of Veterinary Surgery, College of Veterinary Medicine and BK21 FOUR Program, Chonnam National University, Gwangju, Republic of Korea.
Biomaterial R&BD Center, Chonnam National University, Gwangju, Republic of Korea.
出版信息
In Vivo. 2022 Jan-Feb;36(1):233-240. doi: 10.21873/invivo.12696.
BACKGROUND/AIM: Self-defense products that use high-intensity light are being developed. The intense light generated by the high-power light-emitting diodes (LEDs) of such self-defense products causes temporary blindness. However, few studies have been conducted on the visual safety of their devices. We, therefore, evaluated the effects of strong light of a short duration on the eyes of rabbits in this study.
MATERIALS AND METHODS
The right eyes of 15 rabbits were irradiated for 5 s with a lighting device (25 W, 150 lm/W at 700 mA LED) and four eyes of two rabbits were non-irradiated as controls. Changes in the eye structure and function were evaluated before, and immediately, 30 min, 1 h, 24 h, 7 days and 14 days after light irradiation by full-field electroretinogram (ERG), slit-lamp biomicroscopy, and retinal camera. The thickness of the outer nuclear layer of the retina tissue was measured, and histopathological signs of retinal damage were analyzed.
RESULTS
The ERG results showed that night vision was not affected. In day vision, the ERG waveform was temporarily affected immediately after light irradiation; however, it recovered within 24 h. No histopathological signs of damage were observed.
CONCLUSION
Application of high-power LED light with short duration as used for self-defense was found to cause temporary phototoxicity, but safety was confirmed as vision recovered within 24 h.
背景/目的：正在开发使用高强度光的自卫产品。此类自卫产品的大功率发光二极管 (LED) 产生的强光会导致暂时失明。然而，针对此类设备的视觉安全性，研究甚少。因此，我们在这项研究中评估了短时间强光对兔子眼睛的影响。
材料与方法
使用照明设备（25 W，700 mA LED 时为 150 lm/W）对 15 只兔子的右眼照射 5 s，2 只兔子的 4 只眼睛作为对照不进行照射。在光照射前、即刻、30 分钟、1 小时、24 小时、7 天和 14 天后，通过全视野视网膜电图 (ERG)、裂隙灯生物显微镜和视网膜相机评估眼部结构和功能的变化。测量视网膜外核层的厚度，并分析视网膜损伤的组织病理学征象。
结果
ERG 结果表明夜视不受影响。在日视下，ERG 波形在光照射后立即受到暂时影响，但在 24 小时内恢复。未观察到组织病理学损伤迹象。
结论
发现用作自卫的短时间高功率 LED 光会引起暂时的光毒性，但在 24 小时内视力恢复，安全性得到确认。
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