Halstead Susan K, Gourlay Dawn, Willison Hugh J
Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Methods Mol Biol. 2022;2460:183-191. doi: 10.1007/978-1-0716-2148-6_11.
Glycolipids cluster in plasma membranes to form heterogenous patches. Whereas lectins and antibodies have been conventionally viewed as binding a single oligosaccharide head group, and assayed accordingly, it is increasingly evident that cis-interactions between glycan headgroups may form unique molecular shapes that either enhance or attenuate binding of antibodies and other proteins. Herein we describe a method for assaying antibody binding to heteromeric glycolipid complexes that allows rapid, simple, inexpensive and high-throughput assessment of binding events, focusing on autoantibodies present in human serum.
糖脂在质膜中聚集形成异质斑块。虽然凝集素和抗体传统上被视为结合单个寡糖头部基团，并据此进行检测，但越来越明显的是，聚糖头部基团之间的顺式相互作用可能形成独特的分子形状，从而增强或减弱抗体和其他蛋白质的结合。在此，我们描述了一种检测抗体与异聚糖脂复合物结合的方法，该方法能够快速、简单、廉价且高通量地评估结合事件，重点关注人血清中存在的自身抗体。
