National and Kapodistrian University of Athens, Laboratory for the Research of Musculoskeletal Disorders, Athens, Greece.
European University Cyprus, Department of Life Sciences, Nicosia CY, Cyprus.
Adv Exp Med Biol. 2021;1338:55-66. doi: 10.1007/978-3-030-78775-2_8.
Acute lymphoblastic leukemia is the most common childhood malignancy. Rhabdomyosarcoma, on the other hand, is a rare type of malignancy which belongs to the primitive neuroectodermal family of tumors. The aim of the present study was to use computational methods in order to examine the similarities and differences of the two different tumors using two cell lines as a model, the T-cell acute lymphoblastic leukemia CCRF-CEM and rhabdomyosarcoma TE-671, and, in particular, similarities of the metabolic pathways utilized by two different cell types in vitro. Both cell lines were studied using microarray technology. Differential expression profile has revealed genes with similar expression, suggesting that there are common mechanisms between the two cell types, where some of these mechanisms are preserved from their ancestor embryonic cells. Expression of identified species was modeled using known functions, in order to find common patterns in metabolism-related mechanisms. Species expression manifested very interesting dynamics, and we were able to model the system with elliptical/helical functions. We discuss the results of our analysis in the context of the commonly occurring genes between the two cell lines and the respective participating pathways as far as extracellular signaling and cell cycle regulation/proliferation are concerned. In the present study, we have developed a methodology, which was able to unravel some of the underlying dynamics of the metabolism-related species of two different cell types. Such approaches could prove useful in understanding the mechanisms of tumor ontogenesis, progression, and proliferation.
急性淋巴细胞白血病是最常见的儿童恶性肿瘤。另一方面，横纹肌肉瘤是一种罕见的恶性肿瘤，属于原始神经外胚层肿瘤家族。本研究的目的是使用计算方法，通过使用两种细胞系作为模型，即 T 细胞急性淋巴细胞白血病 CCRF-CEM 和横纹肌肉瘤 TE-671，来检查两种不同肿瘤的异同，特别是两种不同细胞类型在体外利用代谢途径的相似性。两种细胞系均采用微阵列技术进行研究。差异表达谱揭示了具有相似表达的基因，这表明两种细胞类型之间存在共同的机制，其中一些机制是从其祖先胚胎细胞中保留下来的。使用已知功能对鉴定出的物种的表达进行建模，以找到与代谢相关机制中的常见模式。物种表达表现出非常有趣的动态，我们能够使用椭圆/螺旋函数对系统进行建模。我们在两种细胞系之间共同出现的基因以及各自参与的途径（如细胞外信号和细胞周期调节/增殖）的背景下讨论了我们分析的结果。在本研究中，我们开发了一种方法，能够揭示两种不同细胞类型的代谢相关物种的一些潜在动态。这些方法可能有助于理解肿瘤发生、进展和增殖的机制。
