School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China; Jiangsu Key Laboratory of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, China.
Sci Total Environ. 2022 Apr 1;815:152678. doi: 10.1016/j.scitotenv.2021.152678. Epub 2021 Dec 29.
Biofilm sequencing batch reactor (BSBR) can achieve efficient phosphate (P) removal and enrichment, but its process performance and metabolic mechanisms for P removal and enrichment of municipal wastewater remain largely unclear. In the present study, we assessed the P removal and enrichment of municipal wastewater at influent P concentrations of 2.5 mg/L and 10 mg/L. The efficiency of P removal and enzyme activity in polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs) were compared, and the growth and metabolic characteristics of dominant PAOs and GAOs at different influent P concentrations were studied with the macro-sequencing technology. The results showed that the P recovery efficiencies were 70.03% and 76.19% when the influent P concentration was 2.5 mg/L and 10 mg/L in BSBR, respectively, and the maximum P concentration of recovery liquid was 81.29 mg/L and 173.12 mg/L, respectively. There were no phosphate kinase (PPK) and phosphate hydrolase (PPX) in extracellular polymeric substances (EPS). The dominant PAOs were Candidatus_Contendobacter, Dechloromonas, and Flavobacterium, and the dominant GAO was Candidatus_Competibacter. The abundance of Candidatus_Contendobacter was the highest with the most potential contribution to P removal. PAOs had competitive advantages in carbon (C) source uptake, glycogen metabolism, P metabolism, and adenosine triphosphate (ATP) metabolism. HMP was unique to PAOs, EMP had the highest abundance in glycogen metabolism, and ED was contained in PAOs of BSBR. These results indicated that BSBR provided sufficient reducing power and ATP for PAOs through different glycogen decomposition pathways to promote P uptake and obtained competitive advantages in P metabolism, C source uptake, and ATP utilization to achieve efficient P removal and enrichment. Collectively, our current findings provided valuable insights into the P removal and enrichment mechanism of BSBR in municipal sewage.
生物膜序批式反应器（BSBR）可以实现高效的磷（P）去除和富集，但对于城市污水中 P 的去除和富集的工艺性能和代谢机制，仍知之甚少。本研究在进水 P 浓度为 2.5mg/L 和 10mg/L 的条件下，评估了城市污水的 P 去除和富集效果。比较了聚磷菌（PAOs）和糖原积累菌（GAOs）的 P 去除效率和酶活性，利用宏基因组测序技术研究了不同进水 P 浓度下优势 PAOs 和 GAOs 的生长和代谢特性。结果表明，BSBR 进水 P 浓度为 2.5mg/L 和 10mg/L 时，P 回收率分别为 70.03%和 76.19%，最大回收液 P 浓度分别为 81.29mg/L 和 173.12mg/L。胞外聚合物（EPS）中不存在磷酸激酶（PPK）和磷酸水解酶（PPX）。优势 PAOs 为 Candidatus_Contendobacter、Dechloromonas 和 Flavobacterium，优势 GAO 为 Candidatus_Competibacter。Candidatus_Contendobacter 的丰度最高，对 P 去除的贡献最大。PAOs 在碳（C）源摄取、糖原代谢、P 代谢和三磷酸腺苷（ATP）代谢方面具有竞争优势。HMP 是 PAOs 特有的，EMP 在糖原代谢中丰度最高，ED 存在于 BSBR 的 PAOs 中。这些结果表明，BSBR 通过不同的糖原分解途径为 PAOs 提供了足够的还原力和 ATP，以促进 P 的摄取，并在 P 代谢、C 源摄取和 ATP 利用方面获得竞争优势，从而实现高效的 P 去除和富集。总之，本研究结果为 BSBR 处理城市污水的 P 去除和富集机制提供了有价值的见解。
