Kirschfink M, Borsos T
Mol Immunol. 1987 Mar;24(3):253-7. doi: 10.1016/0161-5890(87)90143-x.
IgG anti-cell antibodies are inefficient in inducing cell lysis by complement. C mediated lysis by anti-cell IgG can be augmented by the use of C fixing anti-antibody. We have studied augmentation of rabbit anti-Forssman IgG, rabbit anti-methotrexate IgG and rabbit anti-folinic acid IgG antibodies by rabbit anti-allotype IgG antibodies. Large variability (from a low of 2 to a high of over 100) was found in augmentation efficiency even with the same anti-allotype antibody; the variability was primarily the function of the anti-hapten antibody. It was concluded that quantitative studies on anti-hapten IgG production relying on augmented hemolysis may lead to misleading conclusions.
IgG抗细胞抗体在通过补体诱导细胞裂解方面效率不高。抗细胞IgG介导的补体介导裂解可通过使用补体固定抗抗体来增强。我们研究了兔抗异型IgG抗体对兔抗福斯曼IgG、兔抗甲氨蝶呤IgG和兔抗亚叶酸IgG抗体的增强作用。即使使用相同的抗异型抗体，增强效率也存在很大差异（从低至2到高达100以上）；这种差异主要是抗半抗原抗体的作用。得出的结论是，依赖增强溶血的抗半抗原IgG产生的定量研究可能会导致误导性结论。
