Department of Chemical Engineering, Faculty of Engineering, University of Guilan, P. O. Box 41635-3756, Rasht, Iran.
Department of Chemical Engineering, Faculty of Engineering, University of Guilan, P. O. Box 41635-3756, Rasht, Iran.
J Biotechnol. 2022 Jan 20;344:57-69. doi: 10.1016/j.jbiotec.2021.12.013. Epub 2021 Dec 30.
The present work was conducted to investigate the immobilization of lipase from Penicillium sp. onto three modified bentonites by simple adsorption and crosslinking methods. The composites were characterized by FTIR, SEM and BET. The free and bentonite-supported lipase was evaluated in terms of operational and storage stability and pH and thermal activity and stability. The kinetic parameters were also evaluated. The results show that all immobilized enzymes had better thermal and pH stability compared to free enzymes. Among the immobilized enzymes, GDU-bent-lipase had more efficient performance in thermal (38% of its initial activity within 24 h at 65 °C), operational (70% residual activity after 9 cycles), storage stability (70.14% of its initial activities at 4 °C for 21 days), and kinetic properties (effectiveness factor 0.79 relative to free enzyme) than free and other immobilized enzymes. The adsorption isotherm was modeled by Langmuir, Freundlich and Temkin isotherms which Langmuir isotherm indicated a better fit of the experimental adsorption data. To the best of our knowledge, this is the first comparative report about the immobilization of lipase Produced by Penicillium sp., isolated from olive mill wastewater, and the most comprehensive study about the immobilization of lipase onto several supports.
本工作旨在通过简单的吸附和交联方法研究脂肪酶从青霉属固定到三种改性膨润土上。用 FTIR、SEM 和 BET 对复合材料进行了表征。从操作和储存稳定性以及 pH 和热活性和稳定性方面评价了游离酶和膨润土负载酶。还评估了动力学参数。结果表明，所有固定化酶的热稳定性和 pH 稳定性均优于游离酶。在固定化酶中，GDU-膨润土-脂肪酶在热稳定性（65°C 下 24 小时内初始活性的 38%）、操作稳定性（9 个循环后剩余 70%的活性）、储存稳定性（4°C 下 21 天初始活性的 70.14%）和动力学特性（相对于游离酶的效能因子为 0.79）方面的性能优于游离酶和其他固定化酶。吸附等温线通过 Langmuir、Freundlich 和 Temkin 等温线进行了模拟，Langmuir 等温线表明实验吸附数据拟合更好。据我们所知，这是首次对从橄榄加工厂废水中分离出的青霉属产生的脂肪酶进行固定化的比较报告，也是对几种载体固定化脂肪酶的最全面研究。
