使用粗制酸性橄榄渣油生产结构化脂质、乳化剂和生物柴油的综合生物工艺。
Integrated bioprocess for structured lipids, emulsifiers and biodiesel production using crude acidic olive pomace oils.
机构信息
Instituto Superior de Agronomia, Universidade de Lisboa, LEAF - Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Lisbon, Portugal.
Instituto Superior de Agronomia, Universidade de Lisboa, LEAF - Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Lisbon, Portugal; Institute of Technology and Research (ITP), Avenida Murilo Dantas 300 - Farolândia, Aracaju, Brazil; Tiradentes University (UNIT), Avenida Murilo Dantas 300 - Farolândia, Aracaju, Brazil.
出版信息
Bioresour Technol. 2022 Feb;346:126646. doi: 10.1016/j.biortech.2021.126646. Epub 2021 Dec 30.
Olive pomace oil (OPO), a by-product of olive oil industry, is directly consumed after refining. The novelty of this study consists of the direct use of crude high acidic OPO (3.4-20% acidity) to produce added-value compounds, using sn-1,3-regioselective lipases: (i) low-calorie dietetic structured lipids (SL) containing caprylic (C8:0) or capric (C10:0) acids by acidolysis or interesterification with their ethyl esters, (ii) fatty acid methyl esters (FAME) for biodiesel, and (iii) sn-2 monoacylglycerols (emulsifiers), as by-product of FAME production by methanolysis. Immobilized Rhizomucor miehei lipase showed similar activity in acidolysis and interesterification for SL production (yields: 47.8-53.4%, 7 h, 50℃) and was not affected by OPO acidity. Batch operational stability decreased with OPO acidity, but it was at least three-fold in interesterification that in acidolysis. Complete conversion of OPO into FAME and sn-2 monoacylglycerols was observed after 3 h-transesterification (glycerol stepwise addition) and lipase deactivation was negligeable after 11 cycles.
橄榄油渣油（OPO）是橄榄油工业的副产品，精炼后直接食用。本研究的新颖之处在于直接使用粗高酸 OPO（3.4-20%酸度），通过 sn-1,3-区域选择性脂肪酶生产附加值化合物：（i）通过酸解或与它们的乙酯进行酯交换，生成含有辛酸（C8:0）或癸酸（C10:0）的低热量饮食结构脂质（SL），（ii）脂肪酸甲酯（FAME）用于生物柴油，（iii）sn-2 单酰基甘油（乳化剂），作为甲酯化生产 FAME 的副产物。固定化米根霉脂肪酶在 SL 生产的酸解和酯交换中表现出相似的活性（产率：47.8-53.4%，7 h，50℃），不受 OPO 酸度的影响。分批操作稳定性随 OPO 酸度的降低而降低，但在酯交换中的稳定性至少是酸解中的三倍。经过 3 h 酯交换（甘油逐步添加）和脂肪酶失活后，OPO 完全转化为 FAME 和 sn-2 单酰基甘油，在 11 次循环后几乎可以忽略不计。
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