Department of Oral Pathology, Peshawar Dental College, Riphah International University, Islamabad, Pakistan.
Department of Pathology, Peshawar Medical College, Riphah International University,Islamabad , Pakistan.
J Pak Med Assoc. 2021 Oct;71(10):2364-2368. doi: 10.47391/JPMA.1133.
To detect antibodies against p53 in serum of patients of oral squamous cell carcinoma and oral potentially malignant disorders compared to healthy individuals.
The descriptive cross-sectional study was conducted at the departments of Oral and Maxillofacial Surgery, Peshawar Dental College, Peshawar, Khyber College of Dentistry, Peshawar, and the Pakistan Institute of Medical Sciences, Islamabad, Pakistan, from April, 2016 to March, 2017, and comprised serum of oral squamous cell carcinoma patients in group A, oral potentially malignant disorder patients in group B and healthy individuals in group C. Serum concentrations of anti-p53 antibodies were determined by enzyme-linked immunosorbent assay. Data was analysed using SPSS 20.
Of the180 subjects, there were 60(33.3%) in each of the three groups. Circulating serum p53 antibodies were detected in 59(98.3%) cases in group A, 58(96.6%) in group B and in 20(33.3%) individuals in group C (p=0.001).
The expression of circulating p53 antibodies in serum samples of squamous cell carcinoma and oral potentially malignant disorders patients indicates the potential to be a probable candidate for being taken as a diagnostic or prognostic biomarker.
与健康个体相比，检测口腔鳞状细胞癌和口腔潜在恶性疾病患者血清中的 p53 抗体。
本研究为 2016 年 4 月至 2017 年 3 月在巴基斯坦白沙瓦牙科学院口腔颌面外科、白沙瓦牙科学院和巴基斯坦伊斯兰堡巴基斯坦医学科学院进行的描述性横断面研究，纳入了 A 组口腔鳞状细胞癌患者、B 组口腔潜在恶性疾病患者和 C 组健康个体的血清。采用酶联免疫吸附试验检测抗 p53 抗体的血清浓度。采用 SPSS 20 进行数据分析。
180 名受试者中，每组各有 60 名（33.3%）。A 组 59 例（98.3%）、B 组 58 例（96.6%）和 C 组 20 例（33.3%）患者血清中检测到循环血清 p53 抗体（p=0.001）。
在口腔鳞状细胞癌和口腔潜在恶性疾病患者的血清样本中，循环 p53 抗体的表达表明其可能成为一种有希望的诊断或预后生物标志物。
