Barbui T, Buelli M, Cortelazzo S, Viero P, De Gaetano G
Am J Med. 1987 Aug;83(2):265-8. doi: 10.1016/0002-9343(87)90696-6.
Thirty-two patients with thrombocythemia associated with myeloproliferative syndromes were selected on the basis of normal bleeding time and absence of hemorrhagic or thrombotic history. Twenty-five control subjects were studied simultaneously. They were all given a single intravenous infusion of 500 mg of aspirin (lysine acetylsalicylate), and bleeding time was measured two hours later. Both in the control group and in the patient group, aspirin significantly prolonged the bleeding time, but the average prolongation was significantly more pronounced in the patients. In comparison with the control subjects, the patients had a statistically significant reduction of platelet serotonin content and no difference in the production of platelet lipoxygenase derivative 12-HETE or plasma von Willebrand factor properties. Fourteen patients had abnormal platelet aggregation in response to adenosine diphosphate, adrenaline (epinephrine), or collagen. In six of them, all with very low serotonin content, the bleeding time was prolonged above the upper limit of the post-aspirin values in the control group. Thus, cyclooxygenase inhibition by aspirin unmasked a bleeding tendency in patients with a severe reduction in platelet dense bodies content. These findings might be relevant in relation to the use of antiplatelet drugs.
基于出血时间正常且无出血或血栓形成病史，选取了32例与骨髓增殖性综合征相关的血小板增多症患者。同时研究了25名对照受试者。他们均接受了500毫克阿司匹林（赖氨酸乙酰水杨酸）的单次静脉输注，并于两小时后测量出血时间。在对照组和患者组中，阿司匹林均显著延长了出血时间，但患者组的平均延长更为显著。与对照受试者相比，患者的血小板血清素含量在统计学上显著降低，而血小板脂氧合酶衍生物12 - HETE的产生或血浆血管性血友病因子特性无差异。14例患者对二磷酸腺苷、肾上腺素或胶原的血小板聚集反应异常。其中6例患者血清素含量极低，其出血时间延长至对照组阿司匹林后值的上限以上。因此，阿司匹林对环氧化酶的抑制揭示了血小板致密体含量严重降低患者的出血倾向。这些发现可能与抗血小板药物的使用有关。
