Steinberg K K, Rogers T N
Ann Clin Lab Sci. 1987 Jul-Aug;17(4):241-50.
Clinical laboratory tests are increasingly being used to evaluate individuals for osteoporosis and other metabolic bone diseases. Serum bone alkaline phosphatase (AP) [EC 3.1.3.1, orthophosphoric-monoester phosphohydrolase (alkaline optimum)] and osteocalcin are used to assess osteoblastic activity. Although methods for assessing relative amounts of AP isoenzymes continuously appear in the literature, no single method is satisfactory for quantification. Polyacrylamide gel electrophoresis with densitometric scanning combined with two-point heat inactivation was used to obtain quantitative values for AP isoenzymes. Serum bone AP concentrations correlated positively and significantly with serum osteocalcin concentrations obtained by radioimmunoassay for women. Men had significantly higher total alkaline phosphatase and bone AP than women, whereas liver AP concentrations did not differ between the two groups. Bone AP correlated negatively and significantly with age in men, but not women. Osteocalcin concentrations tended to be higher in men, but not significantly.
临床实验室检测越来越多地用于评估个体是否患有骨质疏松症和其他代谢性骨病。血清骨碱性磷酸酶（AP）[EC 3.1.3.1，正磷酸单酯磷酸水解酶（最适碱性）]和骨钙素用于评估成骨细胞活性。尽管评估AP同工酶相对含量的方法不断出现在文献中，但没有一种单一方法能令人满意地进行定量。采用聚丙烯酰胺凝胶电泳结合光密度扫描并结合两点热灭活来获得AP同工酶的定量值。对于女性，血清骨AP浓度与通过放射免疫测定获得的血清骨钙素浓度呈显著正相关。男性的总碱性磷酸酶和骨AP显著高于女性，而两组之间的肝AP浓度没有差异。男性的骨AP与年龄呈显著负相关，而女性则不然。男性的骨钙素浓度往往较高，但差异不显著。
