一种简单有效的冷冻保存方案，适用于具有重要工业价值和模式生物地位的。

A simple and effective cryopreservation protocol for the industrially important and model organism, .

机构信息

Department of Cell Biology, Metabolism and Systems Biology, Noblegen Inc., 2140 East Bank Dr, Peterborough, ON, Canada.

Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada.

出版信息

STAR Protoc. 2021 Dec 15;3(1):101043. doi: 10.1016/j.xpro.2021.101043. eCollection 2022 Mar 18.

DOI:10.1016/j.xpro.2021.101043

PMID:34977684

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8686060/

Abstract

is a source of high-value natural products. A major factor affecting consistent production of biomass is strain stability. Cryopreservation is a leading strategy for cell-line storage that helps ensure process reproducibility. We developed a simple cryopreservation protocol for heterotrophically cultured that enables the recovery of cells after 1 year with a cell viability of ≅80%. This protocol is suitable for labs interested in the long-term preservation of heterotrophic cultures of and related species.

摘要

是高价值天然产物的来源。影响生物量稳定生产的一个主要因素是菌株稳定性。细胞系冻存是确保工艺重现性的主要策略。我们为异养培养的开发了一种简单的冻存方案，可实现细胞的复苏，在经过 1 年的储存后细胞活力约为 80%。该方案适用于对及其相关物种的异养培养长期保存感兴趣的实验室。