Laboratory of Molecular Signaling and Stem Cells Therapy, Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tiantan Xili No. 4, Beijing 100050, China.
Department of geriatric dentistry, Capital Medical University School of Stomatology, Tiantan Xili No. 4, Beijing 100050, China.
Acta Histochem. 2022 Jan;124(1):151835. doi: 10.1016/j.acthis.2021.151835. Epub 2021 Dec 31.
Embryogenesis is modulated by numerous complex signaling cascades, which are essential for normal development. The Hedgehog (Hh) signaling pathway is part of these central cascades. As a homolog of Patched (Ptch)-1, Ptch2 initially did not appear to be as important as Ptch1. Recent reports have revealed that Ptch2 plays a crucial role in ligand-dependent feedback inhibition of Hh signaling in vertebrates. The role of Ptch2 in facial development remains unclear. Here, we investigated the detailed expression pattern of Ptch2 during craniofacial development in murine embryos based on in situ hybridization (ISH) studies of whole-mounts and sections, immunohistochemistry (IHC), and quantitative real-time PCR. We found that both Ptch2 mRNA and protein expression increased in a dynamic pattern in the facial development at mouse embryonic days 11-14.5. Moreover, distinct expression of Ptch2 was observed in the structures of the facial region, such as the tooth germ, Meckel's cartilage, and the follicles of vibrissae. These data, combined with our work in the macrostomia family, suggest that Ptch2 may play a critical role in facial development.
胚胎发生受许多复杂的信号级联调节，这些信号对于正常发育至关重要。Hedgehog（Hh）信号通路是这些核心级联中的一部分。作为 Patched（Ptch）-1 的同源物，Ptch2 最初似乎不如 Ptch1 重要。最近的报告表明，Ptch2 在脊椎动物中配体依赖性反馈抑制 Hh 信号转导中起着至关重要的作用。Ptch2 在面部发育中的作用尚不清楚。在这里，我们通过对整个胚胎和切片的原位杂交（ISH）研究、免疫组织化学（IHC）和定量实时 PCR，研究了 Ptch2 在小鼠胚胎颅面发育过程中的详细表达模式。我们发现 Ptch2 mRNA 和蛋白表达在胚胎发育第 11-14.5 天的面部发育中呈动态增加模式。此外，在面部区域的结构中观察到 Ptch2 的明显表达，例如牙胚、 Meckel 软骨和触须滤泡。这些数据，结合我们在巨口症家族中的工作，表明 Ptch2 可能在面部发育中发挥关键作用。
