School of Food Science and Engineering, South China University of Technology, Guangzhou City, Guangdong Province 510640, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou City, Guangdong Province 510640, China.
Food Res Int. 2022 Jan;151:110886. doi: 10.1016/j.foodres.2021.110886. Epub 2021 Dec 11.
This study characterizes the impact of sdiA on biofilm formation under normal or osmotic stress conditions in Cronobacter sakazakii by constructing a sdiA deletion mutant (ΔsdiA). Here, the downregulation of flagellar assembly-related genes and upregulation of capsular, cellulose and lipopolysaccharide biosynthesis-associated genes in ΔsdiA were observed when compared to the wild type strain (WT) through transcriptomic analysis. Meanwhile, reduced ability of motility, enhanced cell surface hydrophobicity and stronger biofilms with extracellular matrix were observed in WT with deletion of sdiA. Both WT and ΔsdiA formed more biofilm in low osmotic stress medium, while in hyperosmolarity conditions, formation of biofilm was dramatically reduced. Our findings supported that sdiA might suppress biofilm formation of C. sakazakii by regulating biosynthesis of flagellar and extracellular polymeric substances. This study investigates the role of sdiA on biofilm formation in C. sakazakii, and provides the basis for the inhibition of C. sakazakii in food industry and infant-feeding.
本研究通过构建 sdiA 缺失突变体（ΔsdiA），来研究 sdiA 对阪崎克罗诺杆菌在正常或渗透压胁迫条件下生物膜形成的影响。通过转录组分析，与野生型菌株（WT）相比，ΔsdiA 中与鞭毛组装相关的基因下调，而与荚膜、纤维素和脂多糖生物合成相关的基因上调。同时，在 WT 中缺失 sdiA 后，其运动能力降低，细胞表面疏水性增强，胞外基质的生物膜更强。WT 和 ΔsdiA 在低渗透压胁迫培养基中形成更多的生物膜，而在高渗透压条件下，生物膜的形成显著减少。我们的研究结果表明，sdiA 可能通过调节鞭毛和胞外聚合物的生物合成来抑制阪崎克罗诺杆菌的生物膜形成。本研究探讨了 sdiA 对阪崎克罗诺杆菌生物膜形成的作用，为食品工业和婴儿喂养中抑制阪崎克罗诺杆菌提供了依据。
