血管内皮生长因子A及其1型受体在幕上肿瘤中的表达

Expression of Vascular Endothelial Growth Factor A and Its Type 1 Receptor in Supratentorial Neoplasm.

作者信息

Rezaee Hamid, Abbasnia Shadi, Alenabi Anita, Vakili Rosita, Moheghi Nasrin, Tavakol Afshari Jalil, Rezaee Seyed Abdolrahim

机构信息

Neurosurgery Department, Mashhad University of Medical Sciences, Mashhad, Iran.

The first and the second authors contributed equally to this work.

出版信息

Rep Biochem Mol Biol. 2021 Oct;10(3):354-361. doi: 10.52547/rbmb.10.3.354.

DOI:10.52547/rbmb.10.3.354

PMID:34981011

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8718773/

Abstract

BACKGROUND

Vascular endothelial growth factor (VEGF) is one of the primary angiogenesis regulators in solid cancers. Brain solid tumors are life-threatening diseases in which angiogenesis is an important phase of tumor development and progression. In the present study, VEGF-A and VEGF receptor (VEGF-R1) gene expression was evaluated in CNS brain tumors.

METHODS

VEGF-A and VEGF-R1 expression was quantified using real-time PCR on fresh biopsies of 38 supratentorial brain tumors compared to 30 non-tumoral tissues. Then, the correlations were investigated with clinic-pathological and demographic factors of the patients.

RESULTS

PCR product sequencing confirmed the validity of qRT-PCR. Although VEGF-A and VEGF-R1 expression showed increasing trends with the progression of cell proliferation in different stages of astrocytoma, VEGF-R1 did not meet the 95% confidence interval in other brain tumors. An increasing trend in VEGF-A expression and a declining trend in VEGF-R1 expression from Stage I to II were observed in meningioma. VEGF-A and VEGF-R1 expression had no significant correlation with age and gender. Although peritumoral brain edema (PTBE) in astrocytoma was significantly associated with tumor stages, VEGF-A and VEGF-R1 were not correlated with PTBE in meningioma and metastasis.

CONCLUSION

VEGF-A is a valuable factor for the prognosis of PTBE and malignancy in astrocytoma and is helpful in monitoring treatment approaches.

摘要

背景

血管内皮生长因子（VEGF）是实体癌中主要的血管生成调节因子之一。脑实体瘤是威胁生命的疾病，其中血管生成是肿瘤发展和进展的重要阶段。在本研究中，对中枢神经系统脑肿瘤中的VEGF-A和血管内皮生长因子受体（VEGF-R1）基因表达进行了评估。

方法

与30个非肿瘤组织相比，使用实时PCR对38例幕上脑肿瘤的新鲜活检组织中的VEGF-A和VEGF-R1表达进行定量。然后，研究其与患者临床病理和人口统计学因素的相关性。

结果

PCR产物测序证实了qRT-PCR的有效性。虽然在星形细胞瘤不同阶段，VEGF-A和VEGF-R1表达随细胞增殖进展呈增加趋势，但在其他脑肿瘤中VEGF-R1未达到95%置信区间。在脑膜瘤中，观察到从I期到II期VEGF-A表达呈增加趋势，VEGF-R1表达呈下降趋势。VEGF-A和VEGF-R1表达与年龄和性别无显著相关性。虽然星形细胞瘤中的瘤周脑水肿（PTBE）与肿瘤分期显著相关，但VEGF-A和VEGF-R1在脑膜瘤和转移瘤中与PTBE无关。