del Cerro M, Gash D M, Rao G N, Notter M F, Wiegand S J, Sathi S, del Cerro C
Neuroscience. 1987 Jun;21(3):707-23. doi: 10.1016/0306-4522(87)90032-7.
Developing retinas from 13-18-day fetuses and 2-day neonatal Long-Evans rats transplanted into the anterior chamber of adult eyes of the same or different strain (Lewis) survive and differentiate. Light and electron microscopic studies show that the transplants undergo histogenetic differentiation, resulting in the development of neurons and Müller glial cells and formation of nuclear and plexiform layers. Vascular connections develop between the host iris and the retinal transplant. Vessels and nerves, presumably of iridal origin, were seen on the surface of some transplants. Possible manifestations of graft rejection were monitored; signs of tissue rejection in transplants performed in the Long-Evans rats, an outbred strain, were rare and if present they were mild, at least during the survival periods of up to 91 days allowed in these experiments. Transplants into the eyes of Lewis rats were also well tolerated during the survival period. These observations indicate that retinal transplantation to the adult eye of a genetically different host can be successfully achieved and that both embryonic and perinatal retinas are suitable as donor tissue for ocular transplants. The procedure offers ample opportunities for the study of problems related to retinal plasticity.
将13至18日龄胎儿以及出生2天的新生Long-Evans大鼠的发育中的视网膜移植到相同或不同品系（Lewis）成年眼的前房后，这些视网膜能够存活并分化。光镜和电镜研究表明，移植的视网膜会经历组织发生分化，从而发育出神经元和米勒胶质细胞，并形成核层和神经丛层。宿主虹膜与视网膜移植之间会形成血管连接。在一些移植视网膜的表面可见到可能源自虹膜的血管和神经。对移植排斥的可能表现进行了监测；在远交系Long-Evans大鼠中进行的移植，组织排斥迹象罕见，即便出现也很轻微，至少在这些实验所允许的长达91天的存活期内是这样。在存活期内，移植到Lewis大鼠眼中的视网膜也能很好地被耐受。这些观察结果表明，将视网膜移植到基因不同的成年宿主眼中能够成功实现，并且胚胎期和围生期的视网膜都适合作为眼移植的供体组织。该手术为研究与视网膜可塑性相关的问题提供了充足的机会。
