Research & Development, CSL Behring Innovation GmbH, Marburg, Germany.
Research & Development, CSL Behring, Bern, Switzerland.
PLoS One. 2022 Jan 4;17(1):e0262162. doi: 10.1371/journal.pone.0262162. eCollection 2022.
Analysis of convalescent plasma derived from individuals has shown that IgG3 has the most important role in binding to SARS-CoV-2 antigens; however, this has not yet been confirmed in large studies, and the link between binding and neutralization has not been confirmed. By analyzing plasma pools consisting of 247-567 individual convalescent donors, we demonstrated the binding of IgG3 and IgM to Spike-1 protein and the receptor-binding domain correlates strongly with viral neutralization in vitro. Furthermore, despite accounting for only approximately 12% of total immunoglobulin mass, collectively IgG3 and IgM account for approximately 80% of the total neutralization. This may have important implications for the development of potent therapies for COVID-19, as it indicates that hyperimmune globulins or convalescent plasma donations with high IgG3 concentrations may be a highly efficacious therapy.
对个体恢复期血浆的分析表明，IgG3 在与 SARS-CoV-2 抗原结合方面起着最重要的作用；然而，这尚未在大规模研究中得到证实，结合与中和之间的联系也尚未得到证实。通过分析由 247-567 名个体恢复期供体组成的血浆池，我们证明了 IgG3 和 IgM 与 Spike-1 蛋白的结合以及受体结合域与体外病毒中和强烈相关。此外，尽管 IgG3 和 IgM 仅占总免疫球蛋白质量的约 12%，但其总和却约占总中和作用的 80%。这对于开发 COVID-19 的有效疗法可能具有重要意义，因为这表明高 IgG3 浓度的超免疫球蛋白或恢复期血浆捐献可能是一种非常有效的疗法。
